Rabbit anti-Human, Mouse SHARPIN Polyclonal Antibody | anti-SHARPIN antibody

SHARPIN, ID (SHARPIN, SIPL1, Sharpin, Shank-associated RH domain-interacting protein, Shank-interacting protein-like 1) (APC)

Cat. Num. AAA6347319

• Gene Names: SHARPIN; SIPL1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: SHARPIN; Polyclonal Antibody; ID (SHARPIN; SIPL1; Sharpin; Shank-associated RH domain-interacting protein; Shank-interacting protein-like 1) (APC); Shank-interacting protein-like 1; anti-SHARPIN antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SHARPIN. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-SHARPIN antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa240-269 in the central region of human SHARPIN, UniProt Accession #Q9H0F6
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse testis tissue lysates (35ug/lane) usingMBS6005478.This demonstrates that MBS6005478 detected the SHARPIN protein (arrow).)

Related Product Information for anti-SHARPIN antibody

SHARPIN may have a role in normal immune development and control of inflammation (By similarity).

Product Categories/Family for anti-SHARPIN antibody

Antibodies; Proteins

References

1. Jung, J., et al. Mol. Cell. Biochem. 340 (1-2), 161-167 (2010). 2. Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009). 3. Daigo, Y., et al. J. Gastroenterol. Hepatol. 18(6):712-718(2003). 4. Lim, S., et al. Mol. Cell. Neurosci. 17(2):385-397(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 118918415
• NCBI GeneID: 81858
• NCBI Accession #: NP_112236.3
• NCBI GenBank Nucleotide #: NM_030974.3
• UniProt Accession #: Q9H0F6; Q8IXF5; Q8IXF6; Q8N2E7; Q8TB25; Q9BUE4; A6NEG3; C0L3L2; D3DWL3
• Molecular Weight: 39,949 Da
• NCBI Official Full Name: sharpin
• NCBI Official Synonym Full Names: SHANK-associated RH domain interactor
• NCBI Official Symbol: SHARPIN
• NCBI Official Synonym Symbols: SIPL1
• NCBI Protein Information: sharpin; hSIPL1; shank-interacting protein-like 1; shank-associated RH domain-interacting protein
• UniProt Protein Name: Sharpin
• Protein Family: Sharpin
• UniProt Gene Name: SHARPIN
• UniProt Synonym Gene Names: SIPL1; hSIPL1
• UniProt Entry Name: SHRPN_HUMAN

Uniprot Description

Function: Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Ref.10 Ref.11 Ref.12

Subunit structure: Monomer and homodimer. Interacts with SHANK1, EYA1 and EYA2

By similarity. Component of the LUBAC complex (linear ubiquitin chain assembly complex) which consists of SHARPIN, RBCK1 and RNF31. LUBAC has a MW of approximative 600 kDa suggesting a heteromultimeric assembly of its subunits. Associates with the TNF-R1 signaling complex (TNF-RSC) in a stimulation-dependent manner. Ref.10 Ref.11 Ref.12 Ref.13

Subcellular location: Cytoplasm › cytosol Ref.2.

Tissue specificity: Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Up-regulated in various tumor tissues such as kidney, liver, ovary and pancreas tumors. Ref.2

Domain: The Ubiquitin-like domain is required for the interaction with RNF31. Ref.10 Ref.11 Ref.12The RanBP2-type zinc fingers mediate the specific interaction with ubiquitin. Binds preferentially linear polyubiquitin chains and 'Lys-63'-linked polyubiquitin chains over 'Lys-48'-linked polyubiquitin chains. Also binds monoubiquitin. Ref.10 Ref.11 Ref.12

Sequence similarities: Contains 1 RanBP2-type zinc finger.Contains 1 ubiquitin-like domain.

Sequence caution: The sequence BAC11666.1 differs from that shown. Reason: Frameshift at position 334. The sequence BAC53797.1 differs from that shown. Reason: Frameshift at position 218. The sequence BAC53798.1 differs from that shown. Reason: Aberrant splicing.