Mouse Retinal dehydrogenase 1 (ALDH1A1) ELISA Kit | ALDH1A1 elisa kit

Mouse Retinal dehydrogenase 1 (ALDH1A1) ELISA Kit

• Gene Names: ALDH1A1; ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
• Reactivity: Mouse
• Synonyms: Retinal dehydrogenase 1 (ALDH1A1); Mouse Retinal dehydrogenase 1 (ALDH1A1) ELISA Kit; ALDH1A1 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This ALDH1A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ALDH1A1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ALDH1A1 elisa kit

Principle of the assay: ALDH1A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ALDH1A1 antibody and an ALDH1A1-HRP conjugate. The assay sample and buffer are incubated together with ALDH1A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALDH1A1 concentration since ALDH1A1 from samples and ALDH1A1-HRP conjugate compete for the anti-ALDH1A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ALDH1A1 from the sample, fewer sites are left to bind ALDH1A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALDH1A1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ALDH1A1 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 21361176
• NCBI GeneID: 216
• NCBI Accession #: NP_000680.2
• NCBI GenBank Nucleotide #: NM_000689.4
• Molecular Weight: 54,862 Da
• NCBI Official Full Name: retinal dehydrogenase 1
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 1 family, member A1
• NCBI Official Symbol: ALDH1A1
• NCBI Official Synonym Symbols: ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
• NCBI Protein Information: retinal dehydrogenase 1; ALHDII; RALDH 1; ALDH class 1; acetaldehyde dehydrogenase 1; epididymis luminal protein 9; epididymis luminal protein 12; retinaldehyde dehydrogenase 1; aldehyde dehydrogenase 1, soluble; aldehyde dehydrogenase, liver cytosolic; epididymis secretory sperm binding protein Li 53e
• UniProt Protein Name: Retinal dehydrogenase 1
• UniProt Gene Name: ALDH1A1
• UniProt Synonym Gene Names: ALDC; ALDH1; PUMB1; RALDH 1; RalDH1
• UniProt Entry Name: AL1A1_HUMAN