Goat SH3BP1 Polyclonal Antibody | anti-SH3BP1 antibody

Goat anti-SH3BP1 Antibody

Cat. Num. AAA420136

• Gene Names: SH3BP1; ARHGAP43
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: SH3BP1; Polyclonal Antibody; Goat anti-SH3BP1 Antibody; SH3-domain binding protein 1; SH3 domain binding protein 1; OTTHUMP00000028981; SH3BP1 antibody; SH3-domain binding protein 1 antibody; SH3 domain binding protein 1 antibody; OTTHUMP00000028981 antibody; anti-SH3BP1 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: PQPRPRSLASETN
• Sequence Length: 701

• Applicable Applications for anti-SH3BP1 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Immunohistochemistry: In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration, 0.3ug/ml.; Western Blot: Approx 70kDa band observed in Human Lung and Kidney lysates (calculated MW of 75.7kDa according to NP_061830.3). Recommended for use at 1-3ug/ml.; Immunocytochemistry: This antibody has proved to work very well in Caco-2 cells in Elbediwy et al, J. Biol. Chem, 2012 (in press).
• Immunogen: Peptide with sequence C-PQPRPRSLASETN, from the C Terminus of the protein sequence according to NP_061830.3.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

(Staining (1ug/ml) of Human Brain lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

Immunohistochemistry (IHC)

((0.3ug/ml) staining of paraffin embedded Human Kidnay. Cytoplasmic staining of lymphocytes A) in the interstitial parenchyma of the kidney. B) in a glomerulus of the kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 85815829
• NCBI GeneID: 23616
• NCBI Accession #: NP_061830.3
• NCBI GenBank Nucleotide #: NM_018957.3
• Molecular Weight: 50,820 Da
• NCBI Official Full Name: SH3 domain-binding protein 1
• NCBI Official Synonym Full Names: SH3 domain binding protein 1
• NCBI Official Symbol: SH3BP1
• NCBI Official Synonym Symbols: ARHGAP43
• NCBI Protein Information: SH3 domain-binding protein 1
• UniProt Protein Name: SH3 domain-binding protein 1
• Protein Family: SH3 domain-binding protein
• UniProt Gene Name: SH3BP1
• UniProt Synonym Gene Names: 3BP-1
• UniProt Entry Name: 3BP1_HUMAN

Uniprot Description

SH3BP1: Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. This protein binds preferentially to the ABL1 proto-oncogene, SRC and GRB2. Shows GAP activity for Rac-related proteins but not for Rho- or Ras-related proteins. It inhibits PDGF-induced membrane ruffling mediated by Rac. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; GAPs; GAPs, Rac/Rho

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytoplasm

Molecular Function: GTPase activator activity; protein binding; SH3 domain binding

Biological Process: positive regulation of GTPase activity; signal transduction