Rabbit SH2D1A Polyclonal Antibody | anti-SH2D1A antibody

SH2D1A Antibody

Cat. Num. AAA9406668

• Gene Names: SH2D1A; LYP; SAP; XLP; DSHP; EBVS; IMD5; XLPD; MTCP1; XLPD1; SAP/SH2D1A
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: SH2D1A; Polyclonal Antibody; SH2D1A Antibody; DSHP; EBVS; FLJ18687; FLJ92177; anti-SH2D1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total SH2D1A protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 125

• Applicable Applications for anti-SH2D1A antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human SH2D1A.
• Target Name: SH2D1A
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of thymus cell lysate using SH2D1A antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human stomach cancer using SH2D1A antibody at dilution of 1:200 (400x lens))

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat lung using SH2D1A antibody at dilution of 1:200 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using SH2D1A antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-SH2D1A antibody

SH2D1A and SH2D1B are small, adaptor proteins with a single SH2-domain that play important signal transduction roles mediated by the signaling lymphocytic activation molecule (SLAM) family receptors (1). SH2D1A (also called SAP or SLAM-associated protein) is frequently mutated in patients with X-linked lymphoproliferative disease (Duncan's disease), which is characterized by extreme susceptibility to Epstein-Barr virus; approximately 50 different SH2D1A mutations have been reported to date (2-4). The single SH2D1B gene in humans (also called EAT-2 or Ewing's sarcoma's/FLI1-activated transcript 2) is present as a pair of duplicated EAT-2A and EAT-2B genes with identical genomic organization in mouse and rat (5,6).

Product Categories/Family for anti-SH2D1A antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 169234945
• NCBI GeneID: 4068
• NCBI Accession #: NP_001108409.1
• NCBI GenBank Nucleotide #: NM_001114937.2
• UniProt Accession #: O60880; O95383; O95384; O95385; O95386; Q6FGS6; Q9UNR0; A8MSW0
• Molecular Weight: 6,631 Da
• NCBI Official Full Name: SH2 domain-containing protein 1A isoform 2
• NCBI Official Synonym Full Names: SH2 domain containing 1A
• NCBI Official Symbol: SH2D1A
• NCBI Official Synonym Symbols: LYP; SAP; XLP; DSHP; EBVS; IMD5; XLPD; MTCP1; XLPD1; SAP/SH2D1A
• NCBI Protein Information: SH2 domain-containing protein 1A
• UniProt Protein Name: SH2 domain-containing protein 1A
• Protein Family: SH2 domain-containing protein
• UniProt Gene Name: SH2D1A
• UniProt Synonym Gene Names: DSHP; SAP; SLAM-associated protein
• UniProt Entry Name: SH21A_HUMAN

NCBI Description

This gene encodes a protein that plays a major role in the bidirectional stimulation of T and B cells. This protein contains an SH2 domain and a short tail. It associates with the signaling lymphocyte-activation molecule, thereby acting as an inhibitor of this transmembrane protein by blocking the recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to its docking site. This protein can also bind to other related surface molecules that are expressed on activated T, B and NK cells, thereby modifying signal transduction pathways in these cells. Mutations in this gene cause lymphoproliferative syndrome X-linked type 1 or Duncan disease, a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus, with symptoms including severe mononucleosis and malignant lymphoma. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SH2D1A: Inhibitor of the SLAM self-association. Acts by blocking recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to a docking site in the SLAM cytoplasmic region. Mediates interaction between FYN and SLAMF1. May also regulate the activity of the neurotrophin receptors NTRK1, NTRK2 and NTRK3. Interacts with NTRK1, NTRK2 and NTRK3. Interacts with CD84, CD244, LY9, SLAMF1 and FYN. Expressed at a high level in thymus and lung, with a lower level of expression in spleen and liver. Expressed in peripheral blood leukocytes, including T lymphocytes. Tends to be expressed at lower levels in peripheral blood leukocytes in patients with rheumatoid arthritis. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: cytoplasm

Molecular Function: protein binding; SH3/SH2 adaptor activity

Biological Process: positive regulation of signal transduction; cell-cell signaling; cellular defense response; positive regulation of natural killer cell mediated cytotoxicity; humoral immune response

Disease: Lymphoproliferative Syndrome, X-linked, 1