Goat SH2-B/SH2B1 Polyclonal Antibody | anti-SH2B1 antibody
Goat anti-SH2-B/SH2B1 (isoform2) Antibody
Gene Names
SH2B1; PSM; SH2B
Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
SH2-B/SH2B1; Polyclonal Antibody; Goat anti-SH2-B/SH2B1 (isoform2) Antibody; SH2B1; SH2B; SH2-B; DKFZP547G1110; SH2-B homolog; SH2-B alpha signaling protein; SH2-B gamma signaling protein; likely ortholog of mouse SH2 domain-containing putative adapter SH2-B; SH2B adaptor protein 1; DKFZp547G1110; FLJ30542; KIAA1299; PSM; SH2 doma; SH2B1 antibody; SH2B antibody; SH2-B antibody; DKFZP547G1110 antibody; SH2-B homolog antibody; SH2-B alpha signaling protein antibody; SH2-B gamma signaling protein antibody; likely ortholog of mouse SH2 domain-containing putative adapter SH2-B antibody; SH2B adaptor protein 1 antibody; DKFZp547G1110 antibody; FLJ30542 antibody; KIAA1299 antibody; PSM antibody; SH2 domain-containing protein 1B antibody; SH2-B signaling protein antibody; pro-rich; PH and SH2 domain-containing signaling mediator antibody; SH2-B / SH2B1 (isoform2); anti-SH2B1 antibody
Host
Goat
Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
PFGASDCVTDHLP
Sequence Length
671
Applicable Applications for anti-SH2B1 antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Preliminary experiments gave an approx 45-48kDa band in human heart lysate at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of 70.6kDa according to NP_056318.1.
Western Blot: Preliminary experiments gave an approx 45-48kDa band in human heart lysate at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of 70.6kDa according to NP_056318.1.
Immunogen
Peptide with sequence PFGASDCVTDHLP, from the C Terminus of the protein sequence according to NP_056318.2.
Epitope
C Terminus
Note
This antibody is expected to recognise isoform 2 (NP_056318.2) only. Reported variants represent identical protein (NP_001139268.1; NP_056318.2; NP_001139284.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,188 Da
NCBI Official Full Name
SH2B adapter protein 1 isoform 2
NCBI Official Synonym Full Names
SH2B adaptor protein 1
NCBI Official Symbol
SH2B1
NCBI Official Synonym Symbols
PSM; SH2B
NCBI Protein Information
SH2B adapter protein 1
UniProt Protein Name
SH2B adapter protein 1
Protein Family
UniProt Gene Name
SH2B1
UniProt Synonym Gene Names
KIAA1299; SH2B; PSM
UniProt Entry Name
SH2B1_HUMAN
NCBI Description
This gene encodes a member of the SH2-domain containing mediators family. The encoded protein mediates activation of various kinases and may function in cytokine and growth factor receptor signaling and cellular transformation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009]
Uniprot Description
SH2-B-beta: an adaptor protein that specifically activates JAK2 and serve as adapter proteins for JAK1, -2 and -3. YXXL motifs in SH2-Bbeta are phosphorylated by JAK2, JAK1, and platelet-derived growth factor receptor and are required for membrane ruffling.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: cytosol; membrane; nucleus
Molecular Function: protein binding; signal transducer activity
Biological Process: blood coagulation; lamellipodium biogenesis; positive regulation of mitosis; positive regulation of signal transduction
Research Articles on SH2B1
Similar Products
Product Notes
The SH2B1 sh2b1 (Catalog #AAA420824) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SH2-B/SH2B1 (isoform2) Antibody reacts with Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's SH2-B/SH2B1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Preliminary experiments gave an approx 45-48kDa band in human heart lysate at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of 70.6kDa according to NP_056318.1. Researchers should empirically determine the suitability of the SH2B1 sh2b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PFGASDCVTD HLP. It is sometimes possible for the material contained within the vial of "SH2-B/SH2B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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