Rabbit anti-Human SGMS2 Polyclonal Antibody | anti-SGMS2 antibody

SGMS2, CT (SGMS2, SMS2, Phosphatidylcholine:ceramide cholinephosphotransferase 2, Sphingomyelin synthase 2) (PE)

Cat. Num. AAA6347031

• Gene Names: SGMS2; SMS2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SGMS2; Polyclonal Antibody; CT (SGMS2; SMS2; Phosphatidylcholine:ceramide cholinephosphotransferase 2; Sphingomyelin synthase 2) (PE); Sphingomyelin synthase 2; anti-SGMS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SGMS2 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SGMS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336~365 amino acids from the C-terminal region of human SGMS2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SGMS2 Antibody (C-term) western blot analysis in MCF-7,CEM,NCI-H460 cell line lysates (35ug/lane).This demonstrates the SGMS2 antibody detected the SGMS2 protein (arrow).)

Immunohistochemistry (IHC)

(SGMS2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SGMS2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SGMS2 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SGMS2 antibody

Sphingomyelin, a major component of cell and Golgi membranes, is made by the transfer of phosphocholine from phosphatidylcholine onto ceramide, with diacylglycerol as a side product. The protein encoded by this gene is an enzyme that catalyzes this reaction primarily at the cell membrane. The synthesis is reversible, and this enzyme can catalyze the reaction in either direction. The encoded protein is required for cell growth. Three transcript variants encoding the same protein have been found for this gene.

Product Categories/Family for anti-SGMS2 antibody

Antibodies; Enzymes

References

Ternes, P., et al. J. Lipid Res. 50(11):2270-2277(2009). Liu, J., et al. Arterioscler. Thromb. Vasc. Biol. 29(6):850-856(2009). Tani, M., et al. Biochem. Biophys. Res. Commun. 381(3):328-332(2009). Wang, W., et al. Plant Cell 20(11):3163-3179(2008). Yeang, C., et al. Biochim. Biophys. Acta 1781(10):610-617(2008). Villani, M., et al. Biochem. J. 414(1):31-41(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 210147407
• NCBI GeneID: 166929
• NCBI Accession #: NP_001129729.1
• NCBI GenBank Nucleotide #: NM_001136257.1
• UniProt Accession #: Q8NHU3; A8K2S9; B2RA61
• Molecular Weight: 42,280 Da
• NCBI Official Full Name: phosphatidylcholine:ceramide cholinephosphotransferase 2
• NCBI Official Synonym Full Names: sphingomyelin synthase 2
• NCBI Official Symbol: SGMS2
• NCBI Official Synonym Symbols: SMS2
• NCBI Protein Information: phosphatidylcholine:ceramide cholinephosphotransferase 2; SM synthase
• UniProt Protein Name: Phosphatidylcholine:ceramide cholinephosphotransferase 2
• UniProt Gene Name: SGMS2
• UniProt Synonym Gene Names: SMS2
• UniProt Entry Name: SMS2_HUMAN

NCBI Description

Sphingomyelin, a major component of cell and Golgi membranes, is made by the transfer of phosphocholine from phosphatidylcholine onto ceramide, with diacylglycerol as a side product. The protein encoded by this gene is an enzyme that catalyzes this reaction primarily at the cell membrane. The synthesis is reversible, and this enzyme can catalyze the reaction in either direction. The encoded protein is required for cell growth. Three transcript variants encoding the same protein have been found for this gene. There is evidence for more variants, but the full-length nature of their transcripts has not been determined.[provided by RefSeq, Oct 2008]

Uniprot Description

SMS2: Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Plasma membrane SMS2 can also convert phosphatidylethanolamine (PE) to ceramide phosphatidylethanolamine (CPE). Major form in liver. Required for cell growth in certain cell types. Regulator of cell surface levels of ceramide, an important mediator of signal transduction and apoptosis. Regulation of sphingomyelin (SM) levels at the cell surface affects insulin sensitivity. Belongs to the sphingomyelin synthase family.

Protein type: Lipid Metabolism - sphingolipid; Transferase; Membrane protein, integral; Membrane protein, multi-pass; EC 2.7.8.27

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: Golgi apparatus; integral to plasma membrane; plasma membrane; integral to Golgi membrane

Molecular Function: sphingomyelin synthase activity; kinase activity; ceramide cholinephosphotransferase activity

Biological Process: sphingolipid metabolic process; sphingolipid biosynthetic process; sphingomyelin biosynthetic process; phosphorylation