Western Blot (WB)
(Mouse Sgk269 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Sgk269 antibody detected the Sgk269 protein (arrow).)
Rabbit anti-Mouse, Human Sgk269 Polyclonal Antibody | anti-Sgk269 antibody
Sgk269, ID (Peak1, Kiaa2002, Sgk269, Pseudopodium-enriched atypical kinase 1, Sugen kinase 269, Tyrosine-protein kinase SgK269) (Azide free) (HRP)
Gene Names
Peak1; SGK269; AA690169; BE132841; mKIAA2002; 9530046P14; 1110049L02Rik; C230081A13Rik
Reactivity
Mouse, Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Sgk269; Polyclonal Antibody; ID (Peak1; Kiaa2002; Pseudopodium-enriched atypical kinase 1; Sugen kinase 269; Tyrosine-protein kinase SgK269) (Azide free) (HRP); Peak1; Tyrosine-protein kinase SgK269; anti-Sgk269 antibody
Host
Rabbit
Reactivity
Mouse, Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Sgk269. Species Crossreactivity: human
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-Sgk269 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC: 1:10-50
WB: 1:1,000
Applications are based on unconjugated antibody.
WB: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
KLH conjugated synthetic peptide between located within aa 1187-1216 in the central region of mouse Sgk269.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Mouse Sgk269 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Sgk269 antibody detected the Sgk269 protein (arrow).)
Western Blot (WB)
(Mouse Sgk269 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Sgk269 antibody detected the Sgk269 protein (arrow).)
Western Blot (WB)
(Mouse Sgk269 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the Sgk269 antibody detected the Sgk269 protein (arrow).)
Western Blot (WB)
(Mouse Sgk269 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the Sgk269 antibody detected the Sgk269 protein (arrow).)
Immunohistochemistry (IHC)
(Mouse Sgk269 Antibody (Center) (AP14877c)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Sgk269 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Mouse Sgk269 Antibody (Center) (AP14877c)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Sgk269 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Product Categories/Family for anti-Sgk269 antibody
References
Trinidad, J.C., et al. Mol. Cell Proteomics 5(5):914-922(2006). Taniwaki, T., et al. Dev. Growth Differ. 47(3):163-172(2005). Caenepeel, S., et al. Proc. Natl. Acad. Sci. U.S.A. 101(32):11707-11712(2004). Okazaki, N., et al. DNA Res. 11(3):205-218(2004). Zambrowicz, B.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(24):14109-14114(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
191,097 Da
NCBI Official Full Name
pseudopodium-enriched atypical kinase 1
NCBI Official Synonym Full Names
pseudopodium-enriched atypical kinase 1
NCBI Official Symbol
Peak1
NCBI Official Synonym Symbols
SGK269; AA690169; BE132841; mKIAA2002; 9530046P14; 1110049L02Rik; C230081A13Rik
NCBI Protein Information
pseudopodium-enriched atypical kinase 1
UniProt Protein Name
Pseudopodium-enriched atypical kinase 1
UniProt Gene Name
Peak1
UniProt Synonym Gene Names
Kiaa2002; Sgk269
Uniprot Description
Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN ().
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Product Notes
The Sgk269 peak1 (Catalog #AAA6346948) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Sgk269, ID (Peak1, Kiaa2002, Sgk269, Pseudopodium-enriched atypical kinase 1, Sugen kinase 269, Tyrosine-protein kinase SgK269) (Azide free) (HRP) reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Sgk269 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). IHC: 1:10-50 WB: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Sgk269 peak1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sgk269, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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