Rabbit anti-Human SGK1/SGK Polyclonal Antibody | anti-SGK1 antibody

Anti-SGK1/SGK Antibody, Rabbit Polyclonal

Cat. Num. AAA8100759

• Gene Names: SGK1; SGK
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: SGK1/SGK; Polyclonal Antibody; Anti-SGK1/SGK Antibody; Rabbit Polyclonal; SGK1/SGK Antibody; Rabbit PAb; Antigen Affinity Purified; serum/glucocorticoid regulated kinase 1; Anti-SGK Antibody; anti-SGK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human SGK1/SGK
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SGK1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 5-10uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human SGK1/SGK
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human SGK1/SGK, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-SGK1 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:49 kDa Observed band size:49 kDa)

Immunoprecipitation (IP)

(SGK1 was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-SGK1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-SGK1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 49 kDa Observed band size :49 kDa)

Related Product Information for anti-SGK1 antibody

Serum-and glucocorticoid-inducible kinase 1 (SGK1), a protein kinase, shares significant structural similarity with other members of the AGC protein kinase family. The inactive SGK1 structure lacks the alphaC helix and this unique feature makes it distinct from other protein kinases. SGK1 is ubiquitously expressed, which plays a pivotal role in the hormonal regulation of several ion channels, carriers, pumps, and transcription factors or regulators. SGK1 plays an important role in the absorption of Na+ and water through the Na+ channel in the apical membrane of tubular epithelial cells. SGK1 expression is modulated by cell stress and hormones, including gluco-and mineralocorticoids. Compelling evidence suggests that increased SGK1 expression or function is related to the pathogenic stress hypothesis of major depression.

References

Tang Z, et al. (2018) Serum and glucocorticoid-regulated kinase 1 (sgk1) is a predictor of poor prognosis in non-small cell lung cancer, and its dynamic pattern following treatment with sgk1 inhibitor and gamma-ray irradiation was elucidated. Oncol Rep 39 (3): 1505-1515.Martin-Fernandez B, et al. (2014) Relevance of sgk1 in structural, functional and molecular alterations produced by aldosterone in heart. Horm Mol Biol Clin Investig 18 (2): 53-61.Dattilo V, et al. (2020) The emerging role of sgk1 (serum-and glucocorticoid-regulated kinase 1) in major depressive disorder: Hypothesis and mechanisms. Front Genet 11 826.Akhoon BA, et al. (2019) Computational insights into the active structure of sgk1 and its implication for ligand design. Biochimie 165 57-66.

NCBI and Uniprot Product Information

• NCBI GI #: 219521876
• NCBI GeneID: 6446
• NCBI Accession #: NP_001137148.1
• NCBI GenBank Nucleotide #: NM_001143676.1
• UniProt Accession #: O00141; Q5TCN2; Q5TCN3; Q5TCN4; Q5VY65; Q9UN56; B7UUP7; B7UUP8; B7UUP9; B7Z5B2; E1P583
• Molecular Weight: 48,942 Da
• NCBI Official Full Name: serine/threonine-protein kinase Sgk1 isoform 2
• NCBI Official Synonym Full Names: serum/glucocorticoid regulated kinase 1
• NCBI Official Symbol: SGK1
• NCBI Official Synonym Symbols: SGK
• NCBI Protein Information: serine/threonine-protein kinase Sgk1; Sgk1 variant i3; serine/threonine protein kinase SGK; serum/glucocorticoid-regulated kinase 1
• UniProt Protein Name: Serine/threonine-protein kinase Sgk1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: SGK1
• UniProt Synonym Gene Names: SGK
• UniProt Entry Name: SGK1_HUMAN

NCBI Description

This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009]

Uniprot Description

SGK1: serum and glucocorticoid-inducible kinase is an AGC protein kinase of the SGK family. Rapidly induced in response to a variety of stimuli including serum, glucocorticoids, follicle stimulating hormone, osmotic shock and mineralocorticoids. Can be activated via PI3K-dependent and -independent pathways. Plays a role in activating certain potassium, sodium and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. SGK is negatively regulated by ubiquitin modification and proteasome degradation.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; Protein kinase, AGC; EC 2.7.11.1; AGC group; SGK family

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; mitochondrion; cytoplasm; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; potassium channel regulator activity; chloride channel regulator activity; sodium channel regulator activity; calcium channel regulator activity; protein serine/threonine/tyrosine kinase activity; ATP binding

Biological Process: apoptosis; sodium ion transport; positive regulation of transporter activity; protein amino acid phosphorylation; regulation of cell migration; regulation of cell proliferation; regulation of apoptosis; regulation of transcription factor activity; long-term memory; regulation of catalytic activity; regulation of blood pressure; response to stress; regulation of cell growth; transmembrane transport; response to DNA damage stimulus