Western Blot (WB)
(Western blot analysis of extracts from COS-7 cells(uv treatment), using SGK1 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit SGK1 Polyclonal Antibody | anti-SGK1 antibody
SGK1 Antibody
Tissue Specificity: Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
Note: The subcellular localization is controlled by the cell cycle, as well as by exposure to specific hormones and environmental stress stimuli. In proliferating cells, it shuttles between the nucleus and cytoplasm in synchrony with the cell cycle, and in serum/growth factor-stimulated cells it resides in the nucleus. In contrast, after exposure to environmental stress or treatment with glucocorticoids, it is detected in the cytoplasm and with certain stress conditions is associated with the mitochondria. In osmoregulation through the epithelial sodium channel, it can be localized to the cytoplasmic surface of the cell membrane. Nuclear, upon phosphorylation.Cell membrane.
Western Blot (WB)
(Western blot analysis of extracts from COS-7 cells(uv treatment), using SGK1 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from COS-7 cells(uv treatment), using SGK1 Antibody. The lane on the left was treated with blocking peptide.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AF6789) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AF6789) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009]
Uniprot Description
SGK1: serum and glucocorticoid-inducible kinase is an AGC protein kinase of the SGK family. Rapidly induced in response to a variety of stimuli including serum, glucocorticoids, follicle stimulating hormone, osmotic shock and mineralocorticoids. Can be activated via PI3K-dependent and -independent pathways. Plays a role in activating certain potassium, sodium and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. SGK is negatively regulated by ubiquitin modification and proteasome degradation.
Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; Protein kinase, AGC; EC 2.7.11.1; AGC group; SGK family
Chromosomal Location of Human Ortholog: 6q23
Cellular Component: nucleoplasm; endoplasmic reticulum membrane; mitochondrion; cytoplasm; plasma membrane; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; potassium channel regulator activity; chloride channel regulator activity; sodium channel regulator activity; calcium channel regulator activity; protein serine/threonine/tyrosine kinase activity; ATP binding
Biological Process: apoptosis; sodium ion transport; positive regulation of transporter activity; protein amino acid phosphorylation; regulation of cell migration; regulation of cell proliferation; regulation of apoptosis; regulation of transcription factor activity; long-term memory; regulation of catalytic activity; regulation of blood pressure; response to stress; regulation of cell growth; transmembrane transport; response to DNA damage stimulus
Research Articles on SGK1
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Product Notes
The SGK1 sgk1 (Catalog #AAA9613810) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SGK1 Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's SGK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA). IF/ICC: 1:100-1:500 WB: 1:500-1:2000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the SGK1 sgk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SGK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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