Rabbit anti-Human SGF29 Polyclonal Antibody | anti-SGF29 antibody

SGF29, CT (CCDC101, SGF29, SAGA-associated factor 29 homolog, Coiled-coil domain-containing protein 101) (FITC)

Cat. Num. AAA6346859

• Gene Names: CCDC101; SGF29; STAF36
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SGF29; Polyclonal Antibody; CT (CCDC101; SAGA-associated factor 29 homolog; Coiled-coil domain-containing protein 101) (FITC); CCDC101; Coiled-coil domain-containing protein 101; anti-SGF29 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SGF29 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SGF29 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-218 amino acids from the C-terminal region of human SGF29.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SGF29 Antibody (C-term) in MDA-MB435 cell line lysates (35ug/lane).SGF29 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(SGF29 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SGF29 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SGF29 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-SGF29 antibody

Antibodies; CCDC (Coiled-coil Domain-containing)

References

Imielinski, M., et al. Nat. Genet. 41(12):1335-1340(2009). Wang, Y.L., et al. J. Biol. Chem. 283(49):33808-33815(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 19923935
• NCBI GeneID: 112869
• NCBI Accession #: NP_612423.1
• NCBI GenBank Nucleotide #: NM_138414.2
• UniProt Accession #: Q96ES7; Q96MF5
• Molecular Weight: 33,238 Da
• NCBI Official Full Name: SAGA-associated factor 29 homolog
• NCBI Official Synonym Full Names: coiled-coil domain containing 101
• NCBI Official Symbol: CCDC101
• NCBI Official Synonym Symbols: SGF29; STAF36
• NCBI Protein Information: SAGA-associated factor 29 homolog; coiled-coil domain-containing protein 101
• UniProt Protein Name: SAGA-associated factor 29 homolog
• Protein Family: SAGA-associated factor
• UniProt Gene Name: CCDC101
• UniProt Synonym Gene Names: SGF29
• UniProt Entry Name: SGF29_HUMAN

NCBI Description

CCDC101 is a subunit of 2 histone acetyltransferase complexes: the ADA2A (TADA2A; MIM 602276)-containing (ATAC) complex and the SPT3 (SUPT3H; MIM 602947)-TAF9 (MIM 600822)-GCN5 (KAT2A; MIM 602301)/PCAF (KAT2B; MIM 602303) acetylase (STAGA) complex. Both of these complexes contain either GCN5 or PCAF, which are paralogous acetyltransferases (Wang et al., 2008 [PubMed 18838386]).[supplied by OMIM, Apr 2010]

Uniprot Description

CCDC101: Involved in transcriptional regulation, through association with histone acetyltransferase (HAT) SAGA-type complexes like the TFTC-HAT, ATAC or STAGA complexes. Specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3). In the SAGA- type complexes, required to recruit complexes to H3K4me. May be involved in MYC-mediated oncogenic transformation. Belongs to the SGF29 family.

Chromosomal Location of Human Ortholog: 16p11.2

Molecular Function: protein binding; methylated histone residue binding

Biological Process: establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent