Rabbit anti-Human SGEF Polyclonal Antibody | anti-SGEF antibody

SGEF, ID (ARHGEF26, SGEF, Rho guanine nucleotide exchange factor 26, SH3 domain-containing guanine exchange factor) (MaxLight 550)

Cat. Num. AAA6346852

• Gene Names: ARHGEF26; SGEF; CSGEF; HMFN1864
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: SGEF; Polyclonal Antibody; ID (ARHGEF26; Rho guanine nucleotide exchange factor 26; SH3 domain-containing guanine exchange factor) (MaxLight 550); ARHGEF26; SH3 domain-containing guanine exchange factor; anti-SGEF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SGEF.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
• Sequence Length: 871

• Applicable Applications for anti-SGEF antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 378-406 in the central terminal region of human SGEF.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane), usingMBS6013565. This demonstrates that MBS6013565 detected the SGEF protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue, using MBS6013565 followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MBS6013565 for immunohistochemistry.)

Product Categories/Family for anti-SGEF antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

1. Yamada, S., et al. Oncogene 23(35):5901-5911(2004). 2. Ellerbroek, S.M., et al. Mol. Biol. Cell 15(7):3309-3319(2004). 3. Qi, H., et al. Endocrinology 144(5):1742-1752(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 194473700
• NCBI GeneID: 26084
• NCBI Accession #: NP_056410.3
• NCBI GenBank Nucleotide #: NP_056410.3
• UniProt Accession #: Q96DR7
• NCBI Official Full Name: rho guanine nucleotide exchange factor 26 isoform 1
• NCBI Official Synonym Full Names: Rho guanine nucleotide exchange factor 26
• NCBI Official Symbol: ARHGEF26
• NCBI Official Synonym Symbols: SGEF; CSGEF; HMFN1864
• NCBI Protein Information: rho guanine nucleotide exchange factor 26
• UniProt Protein Name: Rho guanine nucleotide exchange factor 26
• UniProt Gene Name: ARHGEF26
• UniProt Synonym Gene Names: SGEF

NCBI Description

This gene encodes a member of the Rho-guanine nucleotide exchange factor (Rho-GEF) family. These proteins regulate Rho GTPases by catalyzing the exchange of GDP for GTP. The encoded protein specifically activates RhoG and plays a role in the promotion of macropinocytosis. Underexpression of the encoded protein may be a predictive marker of chemoresistant disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

Activates RhoG GTPase by promoting the exchange of GDP by GTP. Required for the formation of membrane ruffles during macropinocytosis. Required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB, which induces cytoskeleton rearrangements and promotes bacterial entry.