Rabbit anti-Human SFTPC / Surfactant Protein C Polyclonal Antibody | anti-SFTPC antibody

Rabbit Polyclonal to Human SFTPC / Surfactant Protein C

Cat. Num. AAA248306

• Gene Names: SFTPC; SP-C; PSP-C; SFTP2; SMDP2; BRICD6
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: SFTPC / Surfactant Protein C; Polyclonal Antibody; Rabbit Polyclonal to Human SFTPC / Surfactant Protein C; Anti-SFTPC / Surfactant Protein C Antibody (aa143-173) IHC-plus; SFTPC; BRICD6; PSP-C; Pulmonary surfactant protein C; SMDP2; SFTP2; SP-C; Surfactant protein C; Human SFTPC; Surfactant Protein C; anti-SFTPC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS with 0.09% (w/v) sodium azide
• Sequence Length: 197

• Applicable Applications for anti-SFTPC antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF (1:10 - 1:50), IHC-P (10 ug/ml), WB (1:1000)
• Target Species: Human
• Immunogen Description: KLH-conjugated synthetic peptide from C-terminal region of human SFTPC.
• Immunogen Type: Synthetic peptide
• Immunogen: SFTPC / Surfactant Protein C antibody was raised against kLH-conjugated synthetic peptide from C-terminal region of human SFTPC.
• Antigen Modification: aa143-173
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Human, Lung, Type 2 pneumocytes: Formalin-Fixed Paraffin-Embedded (FFPE))

Immunofluorescence (IF)

(Confocal immunofluorescence of SFTPC Antibody (C-term)with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Western Blot (WB)

(SFTPC Antibody (C-term) Western blot of Jurkat cell line lysates (35 ug/lane). This demonstrates the SFTPC antibody detected the SFTPC protein (arrow).)

NCBI and Uniprot Product Information

• NCBI GI #: 149999608
• NCBI GeneID: 6440
• NCBI Accession #: NP_003009.2
• NCBI GenBank Nucleotide #: NM_003018.3
• UniProt Accession #: P11686; P11687; Q12793; Q7Z5D0; A6XNE4; B2RE00; E9PGX3
• Molecular Weight: 20,361 Da
• NCBI Official Full Name: pulmonary surfactant-associated protein C isoform 1 proprotein
• NCBI Official Synonym Full Names: surfactant protein C
• NCBI Official Symbol: SFTPC
• NCBI Official Synonym Symbols: SP-C; PSP-C; SFTP2; SMDP2; BRICD6
• NCBI Protein Information: pulmonary surfactant-associated protein C; SP5; BRICHOS domain containing 6; pulmonary surfactant apoprotein-2 SP-C; pulmonary surfactant-associated proteolipid SPL(Val)
• UniProt Protein Name: Pulmonary surfactant-associated protein C
• Protein Family: Pulmonary surfactant-associated protein
• UniProt Gene Name: SFTPC
• UniProt Synonym Gene Names: SFTP2; SP-C
• UniProt Entry Name: PSPC_HUMAN

NCBI Description

This gene encodes the pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 2, also called pulmonary alveolar proteinosis due to surfactant protein C deficiency, and are associated with interstitial lung disease in older infants, children, and adults. Alternatively spliced transcript variants encoding different protein isoforms have been identified.[provided by RefSeq, Feb 2010]

Uniprot Description

SFTPC: Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air- liquid interface in the peripheral air spaces. Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2); also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS); also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: multivesicular body; extracellular space

Molecular Function: protein binding; protein homodimerization activity

Biological Process: circadian rhythm; response to retinoic acid; response to hyperoxia; response to cAMP; response to glucocorticoid stimulus; response to glucose stimulus; response to lipopolysaccharide; respiratory gaseous exchange; protein homooligomerization; response to vitamin A

Disease: Pulmonary Fibrosis, Idiopathic; Surfactant Metabolism Dysfunction, Pulmonary, 2