Rabbit SF3B1 Polyclonal Antibody | anti-SF3B1 antibody

SF3B1 antibody - N-terminal region

Cat. Num. AAA3205438

• Gene Names: SF3B1; MDS; PRP10; Hsh155; PRPF10; SAP155; SF3b155
• Reactivity: Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
• Applications: Western Blot
• Purity: Affinity Purified
• Synonyms: SF3B1; Polyclonal Antibody; SF3B1 antibody - N-terminal region; anti-SF3B1 antibody

• Host: Rabbit
• Reactivity: Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
• Clonality: Polyclonal
• Purity/Purification: Affinity Purified
• Form/Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Sequence: Synthetic peptide located within the following region: SARKNRWDETPKTERDTPGHGSGWAETPRTDRGGDSIGETPTPGASKRKS
• Sequence Length: 1304

• Applicable Applications for anti-SF3B1 antibody: Western Blot (WB)
• Homology: Cow: 100%; Dog: 100%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SF3B1
• Preparation and Storage: For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(WB Suggested Anti-SF3B1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Hela cell lysateSF3B1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells)

Related Product Information for anti-SF3B1 antibody

This is a rabbit polyclonal antibody against SF3B1. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: SF3B1 is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Categories/Family for anti-SF3B1 antibody

Polyclonal; RNA Binding Proteins; Chromatin & Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 54112117
• NCBI GeneID: 23451
• NCBI Accession #: NP_036565
• NCBI GenBank Nucleotide #: NM_012433
• UniProt Accession #: O75533
• Molecular Weight: 146kDa
• NCBI Official Full Name: splicing factor 3B subunit 1 isoform 1
• NCBI Official Synonym Full Names: splicing factor 3b subunit 1
• NCBI Official Symbol: SF3B1
• NCBI Official Synonym Symbols: MDS; PRP10; Hsh155; PRPF10; SAP155; SF3b155
• NCBI Protein Information: splicing factor 3B subunit 1
• UniProt Protein Name: Splicing factor 3B subunit 1
• Protein Family: Splicing factor
• UniProt Gene Name: SF3B1
• UniProt Synonym Gene Names: SAP155; SF3b155; SAP 155
• UniProt Entry Name: SF3B1_HUMAN

NCBI Description

This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

SF3B1: subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (bps) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. Phosphorylated during the splicing catalytic steps.

Protein type: Spliceosome; RNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: nucleoplasm; spliceosome; nuclear speck; nucleus; U12-dependent spliceosome

Molecular Function: protein binding; chromatin binding

Biological Process: anterior/posterior pattern formation; nuclear mRNA splicing, via spliceosome; RNA splicing, via transesterification reactions; RNA splicing; gene expression

Disease: Myelodysplastic Syndrome