Western Blot (WB)
((0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat SET/I2 alpha PP2A Polyclonal Antibody | anti-SET antibody
Goat anti-SET/I2 alpha PP2A Antibody
Western Blot: Approx 38kDa band observed in lysates of Human Kidney lysates and of cell lines Daudi and Molt4 (calculated MW of 32.1kDa according to NP_003002.2). Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of Daudi (A) and Molt4 (B) cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of Daudi (A) and Molt4 (B) cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The encoded protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
SET: a potent inhibitor of protein phosphatase 2A involved in apoptosis, transcription, nucleosome assembly and histone binding. The translocation t(6;9)(q21;q34.1) NUP214/CAN is found in some cases of acute undifferentiated leukemia (AUL). Two alternatively spliced isoforms have been described. Isoform 1 and isoform 2 interact directly with each other and with ANP32A within the tripartite INHAT (inhibitor of acetyltransferases) complex. Both isoforms inhibit the acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition.
Protein type: DNA-binding; Oncoprotein; Apoptosis; Nuclear receptor co-regulator; DNA replication; Inhibitor
Chromosomal Location of Human Ortholog: 9q34
Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; nucleoplasm; nucleus; perinuclear region of cytoplasm; protein complex
Molecular Function: DNA binding; histone binding; protein binding; protein phosphatase inhibitor activity; protein phosphatase type 2A regulator activity
Biological Process: DNA replication; negative regulation of catalytic activity; negative regulation of histone acetylation; negative regulation of neuron apoptosis; negative regulation of transcription, DNA-dependent; nucleocytoplasmic transport; nucleosome assembly; nucleosome disassembly; regulation of mRNA stability; regulation of protein phosphatase type 2A activity; viral reproduction
Research Articles on SET
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Product Notes
The SET set (Catalog #AAA420767) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SET/I2 alpha PP2A Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's SET/I2 alpha PP2A can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 38kDa band observed in lysates of Human Kidney lysates and of cell lines Daudi and Molt4 (calculated MW of 32.1kDa according to NP_003002.2). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the SET set for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SAPAAKVSKK ELNS. It is sometimes possible for the material contained within the vial of "SET/I2 alpha PP2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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