Goat Serum, Feline Polyclonal Antibody | anti-SAA1 antibody
Goat anti-Feline Serum, Feline
Gene Names
SAA1; SAA
Reactivity
Feline
Applications
Immunoelectropheresis (IEP)
Synonyms
Serum; Feline; Polyclonal Antibody; Goat anti-Feline Serum; Anti -Serum; anti-SAA1 antibody
Host
Goat
Reactivity
Feline
Clonality
Polyclonal
Specificity
Precipitating polyclonal Goat antiSerum to Cat Serum proteins
Applicable Applications for anti-SAA1 antibody
Immunoelectropheresis (IEP)
Application Notes
Precipitation assays. In immunoelectrophoresis use 2 ul serum or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in 3 mm diameter center well and 2 ul serum samples (neat and serially diluted in 2 mm diameter peripheral wells. Different bleedings of the immunized animals are pooled to obtain a broad spectrum balanced against the varying concentrations of the individual serum protein components.
Source
Pooled whole cat serum and partly purified serum fractions. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Product
Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water.
Species Reactivity Note
Inter-species cross-reactivity is a normal feature of antibodies to animal proteins since homologous proteins of different species frequently share antigenic determinants. This antiSerum has not been adsorbed for such cross-reactivity. Consequently it is not species-specific.
Preparation and Storage
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
Related Product Information for anti-SAA1 antibody
In immunoelectrophoresis against pooled serum and enriched serum proteins fractions precipitation can be observed of not less than 16 individual proteins components. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion (Ouchterlony) to identify the serum protein pattern, or the presence or absence of an individual component. To evaluate the purity of an isolated serum protein including immunoglobulins. Since immunoprecipitation depends on a correct antigen/antibody concentration ratio (zone of equivalence) in the gel medium, the protein analysis by immunoelectrophoresis of serum or any other biological fluid or protein fraction should include different proportions of the reactants. It is not possible to obtain an optimal protein pattern in a single analysis. The electroendosmosis effect of different types of agar on proteins with a different net charge can be used to optimize the resolution power of the test system. Agar Nordic Nr. 2 contains sufficient positively charged ions to optimize the resolution of the proteins in the beta-gamma regions, while the alpha regions will become more dense. Highly purified agar (Agar Nordic nr. 1) with low electroendosmosis favours the resolution of the proteins in the alpha regions, while the major components in the beta-gamma region can still be identified.
Product Categories/Family for anti-SAA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
serum amyloid A protein
NCBI Official Symbol
SAA1
NCBI Official Synonym Symbols
SAA
NCBI Protein Information
serum amyloid A protein
Protein Family
Research Articles on SAA1
Similar Products
Product Notes
The SAA1 (Catalog #AAA645089) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Feline Serum, Feline reacts with Feline and may cross-react with other species as described in the data sheet. AAA Biotech's Serum, Feline can be used in a range of immunoassay formats including, but not limited to, Immunoelectropheresis (IEP). Precipitation assays. In immunoelectrophoresis use 2 ul serum or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in 3 mm diameter center well and 2 ul serum samples (neat and serially diluted in 2 mm diameter peripheral wells. Different bleedings of the immunized animals are pooled to obtain a broad spectrum balanced against the varying concentrations of the individual serum protein components. Researchers should empirically determine the suitability of the SAA1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Serum, Feline, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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