Rabbit SERPINB1 Polyclonal Antibody | anti-SERPINB1 antibody

SERPINB1 Antibody

Cat. Num. AAA9612307

• Gene Names: SERPINB1; EI; LEI; PI2; MNEI; PI-2; HEL57; M/NEI; ELANH2; HEL-S-27
• Reactivity: Human; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: SERPINB1; Polyclonal Antibody; SERPINB1 Antibody; Anti elastase; EI; ELANH2; Epididymis luminal protein 57; HEL57; ILEU_HUMAN; LEI; Leukocyte elastase inhibitor; M/NEI; MNEI; Monocyte/neutrophil elastase inhibitor; Peptidase inhibitor 2; Pi-2; PI2; Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 1; Serpin B1; Serpin peptidase inhibitor clade B (ovalbumin) member 1; serpinb1; anti-SERPINB1 antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SERPINB1 Antibody detects endogenous levels of total SERPINB1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 379

• Applicable Applications for anti-SERPINB1 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human SERPINB1, corresponding to a region within N-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells, using SERPINB1 Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from 293T, using SERPINB1 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-SERPINB1 antibody

Function: Neutrophil serine protease inhibitor that plays an essential role in the regulation of the innate immune response, inflammation and cellular homeostasis (PubMed:30692621). Acts primarily to protect the cell from proteases released in the cytoplasm during stress or infection. These proteases are important in killing microbes but when released from granules, these potent enzymes also destroy host proteins and contribute to mortality. Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3 (PubMed:11747453, PubMed:30692621). Acts also as a potent intracellular inhibitor of GZMH by directly blocking its proteolytic activity (PubMed:23269243). During inflammation, limits the activity of inflammatory caspases CASP1, CASP4 and CASP5 by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation (PubMed:30692621). When secreted, promotes the proliferation of beta-cells via its protease inhibitory function (PubMed:26701651).

Subcellular Location: Secreted. Cytoplasm. Cytoplasmic granule.

Tissue Specificity: In human bone marrow, present in all CD45+ populations. Expression levels are highest in the neutrophil lineage, intermediate in monocytic, and lowest in lymphocytic lineage. Within the neutrophil lineage, expression is highest in promyelocytes.

Subunit Structure: Monomer (PubMed:23269243). Interacts (via C-terminus) with CASP1; CASP4 (via CARD domain) and CASP5; these interactions regulate the activity of inflammatory caspases (PubMed:30692621). Interacts with PRTN3 (PubMed:30692621). Interacts with GZMH (PubMed:23269243).

Similarity: Reactive bond 1 is specific for reaction with chymotrypsin-like protease such as cathepsin G, chymotrypsin, chymase or granzyme H, while reactive bond 2 is specific for reaction with elastase-like protease such as neutrophil elastase, proteinase-3, pancreatic elastase or PSA.Belongs to the serpin family. Ov-serpin subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 13489087
• NCBI GeneID: 1992
• NCBI Accession #: NP_109591.1
• NCBI GenBank Nucleotide #: NP_109591.1
• UniProt Accession #: P30740
• Molecular Weight: Observed Molecular Weight: (Observed)42kD.; Predicted Molecular Weight: (Calculated)43kDa.
• NCBI Official Full Name: leukocyte elastase inhibitor
• NCBI Official Synonym Full Names: serpin family B member 1
• NCBI Official Symbol: SERPINB1
• NCBI Official Synonym Symbols: EI; LEI; PI2; MNEI; PI-2; HEL57; M/NEI; ELANH2; HEL-S-27
• NCBI Protein Information: leukocyte elastase inhibitor
• UniProt Protein Name: Leukocyte elastase inhibitor
• Protein Family: Leukocyte elastase inhibitor
• UniProt Gene Name: SERPINB1
• UniProt Synonym Gene Names: ELANH2; MNEI; PI2; LEI; EI; M/NEI; PI-2
• UniProt Entry Name: ILEU_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

SERPINB1: Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Belongs to the serpin family. Ov-serpin subfamily.

Chromosomal Location of Human Ortholog: 6p25

Cellular Component: extracellular space; membrane; cytoplasm

Molecular Function: serine-type endopeptidase inhibitor activity