Western Blot (WB)
(Western blot analysis of SERPINA4 antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). SERPINA4 (arrow) was detected using the purified Pab.)
Rabbit anti-Human SERPINA4 Polyclonal Antibody | anti-SERPINA4 antibody
SERPINA4, CT (Kallistatin, Kallikrein Inhibitor, Peptidase Inhibitor 4, Serpin A4, PI-4, Serpin A4, KST, PI4) (APC)
Gene Names
SERPINA4; KAL; KST; PI4; KLST; PI-4; kallistatin
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
SERPINA4; Polyclonal Antibody; CT (Kallistatin; Kallikrein Inhibitor; Peptidase Inhibitor 4; Serpin A4; PI-4; KST; PI4) (APC); anti-SERPINA4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SERPINA4 at ~48kD.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-SERPINA4 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-1:50
ELISA: 1:1000
IHC: 1:50-1:100
WB: 1:50-1:100
Applications are based on unconjugated antibody.
ELISA: 1:1000
IHC: 1:50-1:100
WB: 1:50-1:100
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within aa298-328 from the C-terminal region of human SERPINA4.
Conjugate
APC
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of SERPINA4 antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). SERPINA4 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of SERPINA4 antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). SERPINA4 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SERPINA4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SERPINA4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(SERPINA4 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(SERPINA4 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-SERPINA4 antibody
References
1. Chai, K.X., & Chen, L.-M., J. Biol. Chem. 268: 24, 498-24, 505 (1993). 2. Chai, K.X. & Ward, D.C., Genomics 23: 370-378 (1994). 3. Zhou, G.X. & Chao, L., J. Biol. Chem. 267: 25, 873-25, 880 (1992).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,542 Da
NCBI Official Full Name
kallistatin isoform 2
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4
NCBI Official Symbol
SERPINA4
NCBI Official Synonym Symbols
KAL; KST; PI4; KLST; PI-4; kallistatin
NCBI Protein Information
kallistatin; kallikrein inhibitor; peptidase inhibitor 4; protease inhibitor 4 (kallistatin); serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4; serpin A4
UniProt Protein Name
Kallistatin
Protein Family
UniProt Gene Name
SERPINA4
UniProt Synonym Gene Names
KST; PI4; PI-4
UniProt Entry Name
KAIN_HUMAN
Uniprot Description
SERPINA4: Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. Belongs to the serpin family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 14q32.13
Cellular Component: extracellular space
Molecular Function: serine-type endopeptidase inhibitor activity
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Product Notes
The SERPINA4 serpina4 (Catalog #AAA6499034) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SERPINA4, CT (Kallistatin, Kallikrein Inhibitor, Peptidase Inhibitor 4, Serpin A4, PI-4, Serpin A4, KST, PI4) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SERPINA4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-1:50 ELISA: 1:1000 IHC: 1:50-1:100 WB: 1:50-1:100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SERPINA4 serpina4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SERPINA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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