Rabbit anti-Mouse Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) Polyclonal Antibody | anti-SPTLC3 antibody
PE-Linked Polyclonal Antibody to Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3)
Immunohistochemistry: 5-20 ug/mL;1:10-40
Immunocytochemistry: 5-20 ug/mL;1:10-40
Optimal working dilutions must be determined by end user.
Store at 4°C for frequent use.
Aliquot and store at -20°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
NCBI and Uniprot Product Information
NCBI Description
The SPTLC3 gene encodes an isoform of the third subunit of serine palmitoyltransferase (SPT; EC 2.3.1.50), which catalyzes the rate-limiting step of the de novo synthesis of sphingolipids (Hornemann et al., 2006 [PubMed 17023427]). SPT contains 2 main subunits: the common SPTLC1 subunit (MIM 605712) and either SPTLC2 (MIM 605713) or its isoform SPTLC2L (SPTLC3), depending on the tissue in which biosynthesis occurs (Hornemann et al., 2006 [PubMed 17023427]). There are also 2 highly related isoforms of a third subunit, SSSPTA (MIM 613540) and SSSPTB (MIM 610412), that confer acyl-CoA preference of the SPT enzyme and are essential for maximal enzyme activity (Han et al., 2009 [PubMed 19416851]).[supplied by OMIM, Nov 2010]
Uniprot Description
SPTLC3: Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC3-SSSPTA isozyme uses both C14-CoA and C16-CoA as substrates, while the SPTLC1-SPTLC3-SSSPTB has the ability to use a broader range of acyl-CoAs without apparent preference. Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.3.1.50; Endoplasmic reticulum; Membrane protein, integral; Transferase
Chromosomal Location of Human Ortholog: 20p12.1
Cellular Component: endoplasmic reticulum membrane
Molecular Function: serine C-palmitoyltransferase activity
Biological Process: sphingoid biosynthetic process; sphingolipid biosynthetic process
Research Articles on SPTLC3
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Product Notes
The SPTLC3 sptlc3 (Catalog #AAA2079288) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PE-Linked Polyclonal Antibody to Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB),Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western blotting: 0.5-2 ug/mL;1:100-400 Immunohistochemistry: 5-20 ug/mL;1:10-40 Immunocytochemistry: 5-20 ug/mL;1:10-40 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the SPTLC3 sptlc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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