Rabbit FANCI Polyclonal Antibody | anti-FANCI antibody

Anti-Phospho-Ser559 FANCI Antibody

Cat. Num. AAA500071

• Gene Names: FANCI; KIAA1794
• Reactivity: Non-Human Primate
• Applications: Western Blot
• Purity: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
• Synonyms: FANCI; Polyclonal Antibody; Anti-Phospho-Ser559 FANCI Antibody; anti-FANCI antibody

• Host: Rabbit
• Reactivity: Non-Human Primate
• Clonality: Polyclonal
• Specificity: Specific for endogenous levels of the ~150 kDa FANCI protein phosphorylated at Ser559. Immunolabeling is increased when HeLa cells are treated with 256nm UV light for 20 minutes (A) and completely eliminated by treatment with lambda-Ptase (B).
• Purity/Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
• Form/Format: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
• Sequence Length: 1328

• Applicable Applications for anti-FANCI antibody: Western Blot (WB)
• Application Notes: WB: 1:250
• Immunogen Species: Human; Immunogen Information
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser559 of human FANCI.
• Preparation and Storage: Storage at -20 degree C is recommended as aliquots may be taken without freeze/thawing due to presence of 50% glycerol.; Stable for at least 1 year at -20 degree C.

Western Blot (WB)

((B) Western blot of Hela cell lysate treated with UV (~254nm) for 20' showing specific immunolabeling of the ~150 kDa FANCI protein phosphorylated at Ser559 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (lambda-Ptase, 1200 units for 30 min).)

Related Product Information for anti-FANCI antibody

Fanconi anemia, FA, is a rare disorder where cells cannot prevent, repair, or tolerate DNA damage, leading to cancer, progressive bone marrow failure and developmental abnormalities (Ishiai et al., 2008). 16 genes have been implicated in FA, and their products constitute a common FA pathway (Walden and Deans 2014). FANCI (Fanconi anemia complementation group I), is one of two substrates for monoubiquitnation by the FANCL-containing core complex, and is crucial for DNA repair via FAP and intrastrand cross-links (Walden and Deans, 2014). Phosphorylation of ser556 and ser559 are highly conserved and have been determined to be ATM/ATR kinase substrates for the FA-DNA pathway (Smogorzewska et al., 2007). Furthermore, the phosphorylation of ser556 and ser559 have been shown to play key roles in FANCI physically associating with FANCD2, stabilizing the ID2 complex, and supporting its DNA binding and subsequent D2-ubiquitination (Walden and Deans, 2014). Research continues to determine if one site plays more of a role in the DNA repair/damage checkpoint of the FA pathway and stabilization of the ID2 complex.

References

Ishiai M, Kitao H, Smogorzewska A, Tomida J, Kinomura A, Uchida E, Saberi A, Kinoshita E, Kinoshita-Kikuta E, Koike T, Tashiro S, Elledge S, Takata M (2008) FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway. Nat Struct Mol Biol Nov;15(11):1138-46.
Walden H, Deans A (2014) The Fanconi Anemia DNA Repair Pathway: Structural and Functional Insights into a Complex Disorder. Annu Rev Biophys 2014;43:257-78.
Smogorzewska A, Matsuoka S, Vinciguerra P, McDonald ER 3rd, Hurov KE, Luo J, Ballif BA, Gugi SP, Hofmann K, D'Andrea AD, Elledge SJ (2007) Identification of the Fanconi anemia (FANC) I protein, a monoubiquitinated FANCD2 paralog required for crosslink repair. Cell 129(2):289-301.

NCBI and Uniprot Product Information

• NCBI GI #: 164607124
• NCBI GeneID: 55215
• NCBI Accession #: NP_001106849.1
• NCBI GenBank Nucleotide #: NM_001113378.1
• UniProt Accession #: Q9NVI1; Q96JN1; Q96ST0; Q9BT96; A4ZVE4; A5YMH4; A6NJZ0
• Molecular Weight: ~150 kDa
• NCBI Official Full Name: Fanconi anemia group I protein isoform 1
• NCBI Official Synonym Full Names: Fanconi anemia complementation group I
• NCBI Official Symbol: FANCI
• NCBI Official Synonym Symbols: KIAA1794
• NCBI Protein Information: Fanconi anemia group I protein
• UniProt Protein Name: Fanconi anemia group I protein
• Protein Family: Fanconi anemia group I protein
• UniProt Gene Name: FANCI
• UniProt Synonym Gene Names: KIAA1794; Protein FACI

NCBI Description

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group I. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays an essential role in the repair of DNA double-strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. Required for maintenance of chromosomal stability. Specifically binds branched DNA: binds both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Participates in S phase and G2 phase checkpoint activation upon DNA damage.