Western Blot (WB)
(Western blot analysis Septin 1 using Jurkat whole cell lysates)
Rabbit anti-Human Septin 1 Polyclonal Antibody | anti-SEPT1 antibody
Septin 1 Antibody
Gene Names
SEPT1; LARP; SEP1; DIFF6; PNUTL3
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Septin 1; Polyclonal Antibody; Septin 1 Antibody; DIFF6; Differentiation 6 (deoxyguanosine triphosphate triphosphohydrolase); LARP; MGC20394; Peanut like protein 3; Peanut-like protein 3; PNUTL3; SEP1; SEPT1; SEPT1_HUMAN; Septin-1; Serologically defined breast cancer antigen NY BR 24; Serologically defined breast cancer antigen NY-BR-24; anti-SEPT1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Septin 1 antibody detects endogenous levels of total Septin 1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
414
Applicable Applications for anti-SEPT1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human Septin 1
Subcellular Location
Cytoplasm. Cytoplasm > Cytoskeleton. Cytoplasm > Cytoskeleton > Centrosome. Remains at the centrosomes and the nearby microtubules throughout mitosis. Localizes to the midbody during cytokinesis.
Tissue Specificity
Expressed at high levels in lymphoid and hematopoietic tissues.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis Septin 1 using Jurkat whole cell lysates)
Western Blot (WB)
(Western blot analysis Septin 1 using Jurkat whole cell lysates)
Immunofluorescene (IF)
(MBS9602239 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9602239 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-SEPT1 antibody
Function: Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential).
Subunit Structure: Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation (By similarity). Interacts with AURKB.
Similarity: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.
Subunit Structure: Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation (By similarity). Interacts with AURKB.
Similarity: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 41 kDa
Predicted: 42 kDa
Predicted: 42 kDa
NCBI Official Full Name
septin-1
NCBI Official Synonym Full Names
septin 1
NCBI Official Symbol
SEPT1
NCBI Official Synonym Symbols
LARP; SEP1; DIFF6; PNUTL3
NCBI Protein Information
septin-1
UniProt Protein Name
Septin-1
UniProt Gene Name
SEPT1
UniProt Synonym Gene Names
DIFF6; PNUTL3
NCBI Description
This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]
Uniprot Description
Filament-forming cytoskeletal GTPase (). May play a role in cytokinesis (Potential).
Research Articles on SEPT1
Similar Products
Product Notes
The SEPT1 sept1 (Catalog #AAA9602239) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Septin 1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Septin 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the SEPT1 sept1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Septin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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