Rabbit anti-Human SEPHS2 Polyclonal Antibody | anti-SEPHS2 antibody

SEPHS2 antibody

Cat. Num. AAA9407449

• Gene Names: SEPHS2; SPS2; SPS2b
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: SEPHS2; Polyclonal Antibody; SEPHS2 antibody; anti-SEPHS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Sequence Length: 448

• Applicable Applications for anti-SEPHS2 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500-1:3000; Immunofluorescence: 1:100-1:200
• Immunogen Type: Recombinant protein
• Immunogen Description: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 186 and 422 of SEPHS2
• Target Name: SEPHS2
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample (30ug whole cell lysate)A: HeLa S37.5% SDS PAGEPrimary antibody diluted at 1: 1000)

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SEPHS2 antibody at 1: 200 dilution.)

Related Product Information for anti-SEPHS2 antibody

This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. This protein itself contains a selenocysteine residue in its predicted active site. The 3' UTR of the gene has a stem-loop secondary structure called a selenocysteine insertion sequence (SECIS) element, which allows UGA to direct the incorporation of selenocysteine rather than signal a translational stop. Alternatively spliced transcripts have been identified, but their biological validity has not been determined. [provided by RefSeq]

Product Categories/Family for anti-SEPHS2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 34785063
• NCBI GeneID: 22928
• Molecular Weight: 47,305 Da
• NCBI Official Full Name: Selenophosphate synthetase 2
• NCBI Official Synonym Full Names: selenophosphate synthetase 2
• NCBI Official Symbol: SEPHS2
• NCBI Official Synonym Symbols: SPS2; SPS2b
• NCBI Protein Information: selenide, water dikinase 2
• UniProt Protein Name: Selenide, water dikinase 2
• UniProt Gene Name: SEPHS2
• UniProt Synonym Gene Names: SPS2
• UniProt Entry Name: SPS2_HUMAN

NCBI Description

This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. Genes encoding selenocysteine contain a stem-loop secondary structure in their 3' UTR called a selenocysteine insertion sequence (SECIS) element. The protein encoded by this gene contains a selenocysteine residue in its predicted active site. There is a pseudogene for this gene on chromosome 5. [provided by RefSeq, Aug 2013]

Uniprot Description

SEPHS2: Synthesizes selenophosphate from selenide and ATP. Belongs to the selenophosphate synthase 1 family. Class I subfamily.

Protein type: Kinase, other; EC 2.7.9.3; Other Amino Acids Metabolism - selenoamino acid

Chromosomal Location of Human Ortholog: 16p11.2

Molecular Function: selenide, water dikinase activity; ATP binding

Biological Process: selenocysteine biosynthetic process; phosphorylation