Mouse P-Selectin/CD62P ELISA Kit | SELP elisa kit

Mouse P-Selectin/CD62P PicoKine Fast ELISA Kit

Cat. Num. AAA1752731

• Gene Names: Selp; Grmp; CD62P; LECAM3; PADGEM; GMP-140
• Reactivity: Mouse
• Synonyms: P-Selectin/CD62P; Mouse P-Selectin/CD62P PicoKine Fast ELISA Kit; Mouse P-Selectin; P-selectin; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD62P; Selp; Grmp; SELP elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Selp
• Sequence Length: 768

• Samples: Cell culture supernates, serum and plasma (citrate)
• Detection Range: 312pg/ml-20000pg/ml
• Sensitivity: <5pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SELP elisa kit

Principle of the Assay: The Picokine™ Mouse Selp Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Selp with a 96-well strip plate that is pre-coated with antibody specific for Selp. The detection antibody is a biotinylated antibody specific for Selp. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Selp with immunogen: Expression system for standard: NSO; Immunogen sequence: W42-A709. The kit is analytically validated with ready to use reagents. To measure Mouse Selp, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Mouse Selp in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Selp in the sample.!!Background/Introduction: P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1(PSGL-1). The standard product used in this kit is recombinant mouse P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 42-709 amino acids sequence with the molecular mass of 99.1KDa. As a result of glycosylation, the molecular mass of 190-200KDa is revealed by SDS-PAGE.

NCBI and Uniprot Product Information

• NCBI GI #: 266459
• NCBI GeneID: 20344
• UniProt Accession #: Q01102
• NCBI Official Full Name: P-selectin
• NCBI Official Synonym Full Names: selectin, platelet
• NCBI Official Symbol: Selp
• NCBI Official Synonym Symbols: Grmp; CD62P; LECAM3; PADGEM; GMP-140
• NCBI Protein Information: P-selectin
• UniProt Protein Name: P-selectin
• Protein Family: P-selectin
• UniProt Gene Name: Selp
• UniProt Synonym Gene Names: Grmp; GMP-140; LECAM3; PADGEM
• UniProt Entry Name: LYAM3_MOUSE

Uniprot Description

SELP: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the selectin/LECAM family.

Protein type: Membrane protein, integral; Cell adhesion

Cellular Component: nucleoplasm; extracellular space; platelet alpha granule membrane; membrane; cytoplasm; integral to membrane; external side of plasma membrane

Molecular Function: heparin binding; protein binding; lipopolysaccharide binding; carbohydrate binding; glycoprotein binding; calcium-dependent protein binding; sialic acid binding; fucose binding

Biological Process: heterophilic cell adhesion; positive regulation of phosphoinositide 3-kinase cascade; cell-cell adhesion; leukocyte adhesion; positive regulation of cell adhesion; regulation of integrin activation; positive regulation of leukocyte migration; regulation of cellular extravasation; blood vessel morphogenesis; cell adhesion; leukocyte tethering or rolling; inflammatory response; leukocyte migration