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Western Blot (WB) (Western Blot (WB) analysis of SEPHS1 polyclonal antibody)

Rabbit SEPHS1 Polyclonal Antibody | anti-SEPHS1 antibody

SEPHS1 Polyclonal Antibody

Gene Names
SEPHS1; SPS; SELD; SPS1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
SEPHS1; Polyclonal Antibody; SEPHS1 Polyclonal Antibody; Selenide; water dikinase 1; Selenium donor protein 1; Selenophosphate synthase 1; SELD; SPS; SPS1; anti-SEPHS1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SEPHS1 polyclonal antibody detects endogenous levels of SEPHS1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
321
Applicable Applications for anti-SEPHS1 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IF: 1:50-1:100
Immunogen
Recombinant full length Human SEPHS1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SEPHS1 polyclonal antibody)

Western Blot (WB) (Western Blot (WB) analysis of SEPHS1 polyclonal antibody)
Related Product Information for anti-SEPHS1 antibody
SEPHS1 (selenophosphate synthetase 1), also known as SELD, SPS or SPS1, is a 392 amino acid member of the selenophosphate synthetase 1 family and is one of two mammalian homologs of the eubacteria selenophosphate synthetase protein SelD. SelD is an enzyme that generates the selenium donor for the biosynthesis of selenocysteine, an amino acid that is co-translationally incorporated into selenoproteins at in-frame UGA codons. SEPHS1 has a similar function to SelD and specifically catalyzes the formation of selenophosphate (the active selenium donor) from selenide, ATP and H2O. Proper SEPHS1 function depends on a selenium salvage system that recycles L-selenocysteine, thereby providing the substrates for selenophosphate synthesis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 43kDa
NCBI Official Full Name
selenide, water dikinase 1 isoform 3
NCBI Official Synonym Full Names
selenophosphate synthetase 1
NCBI Official Symbol
SEPHS1
NCBI Official Synonym Symbols
SPS; SELD; SPS1
NCBI Protein Information
selenide, water dikinase 1
UniProt Protein Name
Selenide, water dikinase 1
UniProt Gene Name
SEPHS1
UniProt Synonym Gene Names
SELD; SPS; SPS1
UniProt Entry Name
SPS1_HUMAN

NCBI Description

This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. [provided by RefSeq, Sep 2010]

Research Articles on SEPHS1

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Product Notes

The SEPHS1 sephs1 (Catalog #AAA3011581) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SEPHS1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SEPHS1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:2000 IF: 1:50-1:100. Researchers should empirically determine the suitability of the SEPHS1 sephs1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SEPHS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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