Rabbit anti-Human SENP6 Polyclonal Antibody | anti-SENP6 antibody

SENP6, CT (SENP6, KIAA0797, SSP1, SUSP1, Sentrin-specific protease 6, SUMO-1-specific protease 1, Sentrin/SUMO-specific protease SENP6) (PE)

Cat. Num. AAA6346085

• Gene Names: SENP6; SSP1; SUSP1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: SENP6; Polyclonal Antibody; CT (SENP6; KIAA0797; SSP1; SUSP1; Sentrin-specific protease 6; SUMO-1-specific protease 1; Sentrin/SUMO-specific protease SENP6) (PE); Sentrin/SUMO-specific protease SENP6; anti-SENP6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SENP6 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SENP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1083~1112 amino acids from the C-terminal region of human SENP6.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SENP6 polyclonal antibody in HL60 cell lysate. SENP6 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-SENP6 antibody

SENP6 (SUSP1) is a protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. This protein does not seem to be involved in the processing of full-length SUMO proteins to their mature conjugatable forms. SENP6 deconjugates SUMO1 from RXRA, leading to transcriptional activation. It may act preferentially on substrates containing 3 or more SUMO2 or SUMO3 moieties.

Product Categories/Family for anti-SENP6 antibody

Antibodies; Enzymes; Protease (Proteinase; Peptidase)

References

Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Kim, K.I., et al., J. Biol. Chem. 275(19):14102-14106 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 156105703
• NCBI GeneID: 26054
• NCBI Accession #: NP_001093879.1
• NCBI GenBank Nucleotide #: NM_001100409.1
• UniProt Accession #: Q9GZR1; O94891; Q5VUL3; Q5VUL4; Q8TBY4; Q9UJV5; A6NNY9
• Molecular Weight: 126,146 Da
• NCBI Official Full Name: sentrin-specific protease 6 isoform 2
• NCBI Official Synonym Full Names: SUMO1/sentrin specific peptidase 6
• NCBI Official Symbol: SENP6
• NCBI Official Synonym Symbols: SSP1; SUSP1
• NCBI Protein Information: sentrin-specific protease 6; 2810017C20Rik; SUMO-1-specific protease 1; SUMO1/sentrin specific protease 6; sentrin/SUMO-specific protease SENP6
• UniProt Protein Name: Sentrin-specific protease 6
• Protein Family: Sentrin-specific protease
• UniProt Gene Name: SENP6
• UniProt Synonym Gene Names: KIAA0797; SSP1; SUSP1
• UniProt Entry Name: SENP6_HUMAN

NCBI Description

Ubiquitin-like molecules (UBLs), such as SUMO1 (UBL1; MIM 601912), are structurally related to ubiquitin (MIM 191339) and can be ligated to target proteins in a similar manner as ubiquitin. However, covalent attachment of UBLs does not result in degradation of the modified proteins. SUMO1 modification is implicated in the targeting of RANGAP1 (MIM 602362) to the nuclear pore complex, as well as in stabilization of I-kappa-B-alpha (NFKBIA; MIM 164008) from degradation by the 26S proteasome. Like ubiquitin, UBLs are synthesized as precursor proteins, with 1 or more amino acids following the C-terminal glycine-glycine residues of the mature UBL protein. Thus, the tail sequences of the UBL precursors need to be removed by UBL-specific proteases, such as SENP6, prior to their conjugation to target proteins (Kim et al., 2000 [PubMed 10799485]). SENPs also display isopeptidase activity for deconjugation of SUMO-conjugated substrates (Lima and Reverter, 2008 [PubMed 18799455]).[supplied by OMIM, Jun 2009]

Uniprot Description

SENP6: Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Processes preferentially poly-SUMO2 and poly- SUMO3 chains, but does not efficiently process SUMO1, SUMO2 and SUMO3 precursors. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. Involved in chromosome alignment and spindle assembly, by regulating the kinetochore CENPH-CENPI-CENPK complex. Desumoylates PML and CENPI, protecting them from degradation by the ubiquitin ligase RNF4, which targets polysumoylated proteins for proteasomal degradation. Desumoylates also RPA1, thus preventing recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination. Belongs to the peptidase C48 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.22.68

Chromosomal Location of Human Ortholog: 6q14.1

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; SUMO-specific protease activity

Biological Process: protein desumoylation; protein sumoylation; proteolysis