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Western Blot (WB) (Western blot analysis of SENP5 polyclonal antibody in Saos-2 cell lysate. SENP5 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Rabbit anti-Human SENP5 Polyclonal Antibody | anti-SENP5 antibody

SENP5, CT (Sentrin-specific protease 5, Sentrin/SUMO-specific protease SENP5, FKSG45) (Azide free) (HRP)

Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
SENP5; Polyclonal Antibody; CT (Sentrin-specific protease 5; Sentrin/SUMO-specific protease SENP5; FKSG45) (Azide free) (HRP); anti-SENP5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SENP5.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-SENP5 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-1:100
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human SENP5 (KLH).
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SENP5 polyclonal antibody in Saos-2 cell lysate. SENP5 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Western Blot (WB) (Western blot analysis of SENP5 polyclonal antibody in Saos-2 cell lysate. SENP5 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SENP5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SENP5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Product Categories/Family for anti-SENP5 antibody
References
1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
81,310 Da
NCBI Official Full Name
sentrin-specific protease 5
NCBI Official Synonym Full Names
SUMO1/sentrin specific peptidase 5
NCBI Official Symbol
SENP5
NCBI Protein Information
sentrin-specific protease 5; SUMO1/sentrin specific protease 5; sentrin/SUMO-specific protease SENP5
UniProt Protein Name
Sentrin-specific protease 5
Protein Family
UniProt Gene Name
SENP5
UniProt Entry Name
SENP5_HUMAN

Uniprot Description

SENP5: Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. Has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. Required for cell division. Belongs to the peptidase C48 family.

Protein type: Nucleolus; EC 3.4.22.68; Protease

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nucleolus; nucleus

Molecular Function: protein binding; cysteine-type peptidase activity

Biological Process: cellular protein metabolic process; protein sumoylation; cell division; proteolysis; post-translational protein modification; cell cycle

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Product Notes

The SENP5 senp5 (Catalog #AAA6498828) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SENP5, CT (Sentrin-specific protease 5, Sentrin/SUMO-specific protease SENP5, FKSG45) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SENP5 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-1:100 ELISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SENP5 senp5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SENP5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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