Rabbit anti-Human SENP3 Polyclonal Antibody | anti-SENP3 antibody

SENP3, NT (Sentrin-specific Protease 3, Sentrin/SUMO-specific Protease SENP3, SUMO-1 Specific Protease 3, SSP3, SUSP3) (PE)

Cat. Num. AAA6498812

• Gene Names: SENP3; SSP3; Ulp1; SMT3IP1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: SENP3; Polyclonal Antibody; NT (Sentrin-specific Protease 3; Sentrin/SUMO-specific Protease SENP3; SUMO-1 Specific Protease 3; SSP3; SUSP3) (PE); anti-SENP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SENP3.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SENP3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human SENP3 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SENP3 N-term polyclonal antibody in Saos-2 cell lysate. SENP3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SENP3 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-SENP3 antibody

Antibodies; Ubiquitin

References

1. Muller S, et al., Nat Rev Mol Cell Biol. 2001 2(3):202-10. Review. 2. Hochstrasser M. Cell. 2001 107(1):5-8. Review. 3. Kahyo T, et al., Mol Cell. 2001 Sep;8(3):713-8. 4. Yeh ET, et al., Gene. 2000 May 2;248(1-2):1-14. Review. 5. Keane, M.M., et al., Oncogene 18 (22), 3365-3375 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 21361499
• NCBI GeneID: 26168
• NCBI Accession #: NP_056485
• NCBI GenBank Nucleotide #: NM_015670
• UniProt Accession #: Q9H4L4; Q66K15; Q86VS7; Q96PS4; Q9Y3W9
• Molecular Weight: 65,010 Da
• NCBI Official Full Name: sentrin-specific protease 3
• NCBI Official Synonym Full Names: SUMO1/sentrin/SMT3 specific peptidase 3
• NCBI Official Symbol: SENP3
• NCBI Official Synonym Symbols: SSP3; Ulp1; SMT3IP1
• NCBI Protein Information: sentrin-specific protease 3; SUMO-1-specific protease 3; sentrin/SUMO-specific protease 3
• UniProt Protein Name: Sentrin-specific protease 3
• Protein Family: Sentrin-specific protease
• UniProt Gene Name: SENP3
• UniProt Synonym Gene Names: SSP3; SUSP3
• UniProt Entry Name: SENP3_HUMAN

Uniprot Description

SENP3: Protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. Deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability. Deconjugates SUMO2 and SUMO3 from CDCA8. Redox sensor that, when redistributed into nucleoplasm, can act as an effector to enhance HIF1A transcriptional activity by desumoylating EP300. Required for rRNA processing through deconjugation of SUMO2 and SUMO3 from nucleophosmin, NPM1. Binds to SUMO1 and SUMO3. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with EP300, NPM1 and CDCA8. On oxidative stress, SENP3 degradation is blocked by inhibition of its ubiquitination, which stabilizes it as it accumulates in the nucleoplasm. Belongs to the peptidase C48 family.

Protein type: EC 3.4.22.68; Ubiquitin-specific protease; Nucleolus; Protease

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; cysteine-type peptidase activity

Biological Process: proteolysis