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Immunofluorescence (IF) (SENP3 antibody - N-terminal region in HeLa cells using Immunofluorescence.Antibody concentration: 2 ug/mlData submitted by: Michael Matunis, Johns Hopkins UniversitySENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa)

Rabbit SENP3 Polyclonal Antibody | anti-SENP3 antibody

SENP3 antibody - N-terminal region

Gene Names
SENP3; SSP3; Ulp1; SMT3IP1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
Immunofluorescence, Western Blot
Purity
Affinity Purified
Synonyms
SENP3; Polyclonal Antibody; SENP3 antibody - N-terminal region; anti-SENP3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence
Synthetic peptide located within the following region: PPPKPRLKSGGGFGPDPGSGTTVPARRLPVPRPSFDASASEEEEEEEEEE
Sequence Length
574
Applicable Applications for anti-SENP3 antibody
Immunofluorescence (IF), Western Blot (WB)
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human SENP3
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunofluorescence (IF)

(SENP3 antibody - N-terminal region in HeLa cells using Immunofluorescence.Antibody concentration: 2 ug/mlData submitted by: Michael Matunis, Johns Hopkins UniversitySENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa)

Immunofluorescence (IF) (SENP3 antibody - N-terminal region in HeLa cells using Immunofluorescence.Antibody concentration: 2 ug/mlData submitted by: Michael Matunis, Johns Hopkins UniversitySENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa)

Immunohistochemistry (IHC)

(SENP3 antibody - N-terminal region in HeLa cells using Immunofluorescence.SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa)

Immunohistochemistry (IHC) (SENP3 antibody - N-terminal region in HeLa cells using Immunofluorescence.SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa)

Western Blot (WB)

(Sample Type: RatSample Type: 1. Rat Liver (200ug)2. Rat Brain (200ug)3. Rat Testis (200ug)4. Rat Kidney (200ug)5.Rat Liver nuclei (-) no HA-SUMO2-WME6.Rat Liver nuclei (+) incubated with 100 ng HA-SUMO2-WMEPrimary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, PortugalSee Customer Feedback tab for detailed information.)

Western Blot (WB) (Sample Type: RatSample Type: 1. Rat Liver (200ug)2. Rat Brain (200ug)3. Rat Testis (200ug)4. Rat Kidney (200ug)5.Rat Liver nuclei (-) no HA-SUMO2-WME6.Rat Liver nuclei (+) incubated with 100 ng HA-SUMO2-WMEPrimary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, PortugalSee Customer Feedback tab for detailed information.)

Western Blot (WB)

(Sample Type: 1. HeLa cell extract (200ug)2. HeLa cell extract (200ug) incubated with HA-SUMO2-VME*HA-S2-VME-SENP3Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal.SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa)

Western Blot (WB) (Sample Type: 1. HeLa cell extract (200ug)2. HeLa cell extract (200ug) incubated with HA-SUMO2-VME*HA-S2-VME-SENP3Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal.SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa)

Western Blot (WB)

(Sample Type: 1. HeLa cell extract (200ug)Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal.SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa)

Western Blot (WB) (Sample Type: 1. HeLa cell extract (200ug)Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal.SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa)

Western Blot (WB)

(WB Suggested Anti-SENP3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: 721_B cell lysateSENP3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells)

Western Blot (WB) (WB Suggested Anti-SENP3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: 721_B cell lysateSENP3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells)
Related Product Information for anti-SENP3 antibody
This is a rabbit polyclonal antibody against SENP3. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: SENP3 is a protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. SENP3 deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability.
Product Categories/Family for anti-SENP3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
65kDa
NCBI Official Full Name
sentrin-specific protease 3
NCBI Official Synonym Full Names
SUMO specific peptidase 3
NCBI Official Symbol
SENP3
NCBI Official Synonym Symbols
SSP3; Ulp1; SMT3IP1
NCBI Protein Information
sentrin-specific protease 3
UniProt Protein Name
Sentrin-specific protease 3
Protein Family
UniProt Gene Name
SENP3
UniProt Synonym Gene Names
SSP3; SUSP3
UniProt Entry Name
SENP3_HUMAN

NCBI Description

The reversible posttranslational modification of proteins by the addition of small ubiquitin-like SUMO proteins (see SUMO1; MIM 601912) is required for numerous biologic processes. SUMO-specific proteases, such as SENP3, are responsible for the initial processing of SUMO precursors to generate a C-terminal diglycine motif required for the conjugation reaction. They also have isopeptidase activity for the removal of SUMO from high molecular mass SUMO conjugates (Di Bacco et al., 2006 [PubMed 16738315]).[supplied by OMIM, Jun 2009]

Uniprot Description

SENP3: Protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. Deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability. Deconjugates SUMO2 and SUMO3 from CDCA8. Redox sensor that, when redistributed into nucleoplasm, can act as an effector to enhance HIF1A transcriptional activity by desumoylating EP300. Required for rRNA processing through deconjugation of SUMO2 and SUMO3 from nucleophosmin, NPM1. Binds to SUMO1 and SUMO3. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with EP300, NPM1 and CDCA8. On oxidative stress, SENP3 degradation is blocked by inhibition of its ubiquitination, which stabilizes it as it accumulates in the nucleoplasm. Belongs to the peptidase C48 family.

Protein type: EC 3.4.22.68; Ubiquitin-specific protease; Nucleolus; Protease

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; cysteine-type peptidase activity

Biological Process: proteolysis

Research Articles on SENP3

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Product Notes

The SENP3 senp3 (Catalog #AAA3212160) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SENP3 antibody - N-terminal region reacts with Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SENP3 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). Researchers should empirically determine the suitability of the SENP3 senp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: PPPKPRLKSG GGFGPDPGSG TTVPARRLPV PRPSFDASAS EEEEEEEEEE. It is sometimes possible for the material contained within the vial of "SENP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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