Rabbit anti-Mouse Semaphorin 5B (SEMA5B) Polyclonal Antibody | anti-SEMA5B antibody

Polyclonal Antibody to Semaphorin 5B (SEMA5B)

Cat. Num. AAA2006432

• Gene Names: Sema5b; SemG; Semag; AI893641; mKIAA1445
• Reactivity: Mouse
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Semaphorin 5B (SEMA5B); Polyclonal Antibody; Polyclonal Antibody to Semaphorin 5B (SEMA5B); anti-SEMA5B antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against SEMA5B. It has been selected for its ability to recognize SEMA5B in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGSEF-GDAAEYQDCN PQACPVRGAW SCWTAWSQCS ASCGGGHYQR TRSCTSPAPS PGEDICLGLH TEEALCSTQA CPEGWSLWSE WGVCTEDGAQ SRSRSCEELL PGPGACVGNS SQSRPCPYSE IPVILPASSV EETTSCGGFN LIHLIVTGVS CFLVSGLLTL AVYLSCQHCQ RQSQESTL
• Sequence Length: 1057

• Applicable Applications for anti-SEMA5B antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant SEMA5B (Gly836~Leu1013) expressed in E.coli.
• Cross Reactivity: Mouse
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2083433
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Mouse Serum.)

Western Blot (WB)

(Western Blot: Sample: Recombinant SEMA5B, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P. Samples: Mouse Tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 164698462
• NCBI GeneID: 20357
• NCBI Accession #: NP_038689.2
• NCBI GenBank Nucleotide #: NM_013661.2
• UniProt Accession #: Q60519; Q6ZPQ8; Q7TT33; Q8BVE5; B1B1A9
• Molecular Weight: 123,131 Da
• NCBI Official Full Name: semaphorin-5B
• NCBI Official Synonym Full Names: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B
• NCBI Official Symbol: Sema5b
• NCBI Official Synonym Symbols: SemG; Semag; AI893641; mKIAA1445
• NCBI Protein Information: semaphorin-5B
• UniProt Protein Name: Semaphorin-5B
• Protein Family: Semaphorin
• UniProt Gene Name: Sema5b
• UniProt Synonym Gene Names: Kiaa1445; Semag; SemG; Sema G

Uniprot Description

SEMA5B: a single-pass type I membrane protein of the semaphorin family that may act as positive axonal guidance cues. Two alternatively spliced human isoforms have been described.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 16|16 B3

Cellular Component: extracellular space; integral component of membrane; membrane

Biological Process: axon extension; cell differentiation; detection of light stimulus involved in visual perception; multicellular organism development; negative chemotaxis; nervous system development