Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Semaphorin 4B (SEMA4B) Polyclonal Antibody | anti-SEMA4B antibody
Polyclonal Antibody to Semaphorin 4B (SEMA4B)
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Semaphorin 4B (SEMA4B); Polyclonal Antibody; Polyclonal Antibody to Semaphorin 4B (SEMA4B); anti-SEMA4B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against SEMA4B. It has been selected for its ability to recognize SEMA4B in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- LHKAVSVGP RVHIIEELQI FSSGQPVQNL LLDTHRGLLY AASHSGVVQV PMANCSLYRS CGDCLLARDP YCAWSGSSCK HVSLYQPQLA TRPWIQDIEG ASAKDLCSAS SVVSPSFVPT GEKPCEQVQF QPNTVNTLAC PLLSNLATRL WLRNGAPVNA SASCHVLPTG DLLLVGT
MGHHHHHHSGSEF- LHKAVSVGP RVHIIEELQI FSSGQPVQNL LLDTHRGLLY AASHSGVVQV PMANCSLYRS CGDCLLARDP YCAWSGSSCK HVSLYQPQLA TRPWIQDIEG ASAKDLCSAS SVVSPSFVPT GEKPCEQVQF QPNTVNTLAC PLLSNLATRL WLRNGAPVNA SASCHVLPTG DLLLVGT
Sequence Length
837
Applicable Applications for anti-SEMA4B antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant SEMA4B (Leu192~Thr367) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2084903
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:HumanTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:HumanTissue))
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
50,721 Da
UniProt Protein Name
Semaphorin-4B
Protein Family
UniProt Gene Name
SEMA4B
Uniprot Description
SEMA4B iso3: a single-pass type I membrane protein that inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Two alternatively spliced human isoforms have been described.
Protein type: Membrane protein, integral; Cell surface
Chromosomal Location of Human Ortholog: 15q25
Cellular Component: plasma membrane; integral to membrane; synapse
Molecular Function: receptor activity
Biological Process: nervous system development; cell differentiation
Research Articles on SEMA4B
Similar Products
Product Notes
The SEMA4B sema4b (Catalog #AAA2006462) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Semaphorin 4B (SEMA4B) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Semaphorin 4B (SEMA4B) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the SEMA4B sema4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- LHKAVSVGP RVHIIEELQI FSSGQPVQNL LLDTHRGLLY AASHSGVVQV PMANCSLYRS CGDCLLARDP YCAWSGSSCK HVSLYQPQLA TRPWIQDIEG ASAKDLCSAS SVVSPSFVPT GEKPCEQVQF QPNTVNTLAC PLLSNLATRL WLRNGAPVNA SASCHVLPTG DLLLVGT. It is sometimes possible for the material contained within the vial of "Semaphorin 4B (SEMA4B), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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