Western Blot (WB)
(All lanes : Anti-SEMA4G Antibody (Center) at 1:2000 dilutionLane 1: A549 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: HT-29 whole cell lysatesLane 4: SH-SY5Y whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 91 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human SEMA4G Polyclonal Antibody | anti-SEMA4G antibody
SEMA4G Antibody (Center)
Western Blot (WB)
(All lanes : Anti-SEMA4G Antibody (Center) at 1:2000 dilutionLane 1: A549 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: HT-29 whole cell lysatesLane 4: SH-SY5Y whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 91 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-SEMA4G Antibody (Center) at 1:2000 dilutionLane 1: A549 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: HT-29 whole cell lysatesLane 4: SH-SY5Y whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 91 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Deloukas P.,et al.Nature 429:375-381(2004).
NCBI and Uniprot Product Information
NCBI Description
Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
Uniprot Description
SEMA4G: a single-pass type I membrane protein that may play a role in axon guidance.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 10q24.31
Cellular Component: plasma membrane; integral to membrane
Molecular Function: receptor activity
Biological Process: nervous system development; cell differentiation
Research Articles on SEMA4G
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Product Notes
The SEMA4G sema4g (Catalog #AAA9212928) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SEMA4G Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SEMA4G can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB~~1:2000. Researchers should empirically determine the suitability of the SEMA4G sema4g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SEMA4G, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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