Western Blot (WB)
(All lanes : Anti-SEMA4F Antibody (C-term) at 1:1000 dilutionLane 1: rat lung lysatesLane 2: U87-MG whole cell lysatesLane 3: HepG2 whole cell lysatesLane 4: A549 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 84 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit SEMA4F Polyclonal Antibody | anti-SEMA4F antibody
SEMA4F Antibody (C-term)
Gene Names
SEMA4F; S4F; SEMAM; SEMAW; M-SEMA; PRO2353; m-Sema-M
Reactivity
Human, RatPredicted: Mouse
Applications
Western Blot, ELISA
Synonyms
SEMA4F; Polyclonal Antibody; SEMA4F Antibody (C-term); Semaphorin-4F; Semaphorin-M; Sema M; Semaphorin-W; Sema W; SEMAM; SEMAW; anti-SEMA4F antibody
Host
Rabbit
Reactivity
Human, Rat
Predicted: Mouse
Predicted: Mouse
Clonality
Polyclonal
Isotype
Ig
Specificity
This SEMA4F antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 695-709 amino acids from the C-terminal region of human SEMA4F.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
737
Applicable Applications for anti-SEMA4F antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000
Antigen Source
Human
Antigen Region
695-709
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(All lanes : Anti-SEMA4F Antibody (C-term) at 1:1000 dilutionLane 1: rat lung lysatesLane 2: U87-MG whole cell lysatesLane 3: HepG2 whole cell lysatesLane 4: A549 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 84 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-SEMA4F Antibody (C-term) at 1:1000 dilutionLane 1: rat lung lysatesLane 2: U87-MG whole cell lysatesLane 3: HepG2 whole cell lysatesLane 4: A549 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 84 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-SEMA4F antibody
Description: Purified Rabbit Polyclonal Antibody (Pab)
Background: Has growth cone collapse activity against retinal ganglion-cell axons (By similarity).
Background: Has growth cone collapse activity against retinal ganglion-cell axons (By similarity).
References
Encinas J.A.,et al.Proc. Natl. Acad. Sci. U.S.A. 96:2491-2496(1999). Wiemann S.,et al.Genome Res. 11:422-435(2001). Otsuki T.,et al.DNA Res. 12:117-126(2005). Hillier L.W.,et al.Nature 434:724-731(2005). Jang W.,et al.Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83511
NCBI Official Full Name
semaphorin-4F isoform 3
NCBI Official Synonym Full Names
ssemaphorin 4F
NCBI Official Symbol
SEMA4F
NCBI Official Synonym Symbols
S4F; SEMAM; SEMAW; M-SEMA; PRO2353; m-Sema-M
NCBI Protein Information
semaphorin-4F
UniProt Protein Name
Semaphorin-4F
Protein Family
UniProt Gene Name
SEMA4F
UniProt Synonym Gene Names
SEMAM; SEMAW; Sema M; Sema W
NCBI Description
This gene encodes a transmembrane class IV semaphorin family protein, which plays a role in neural development. This gene may be involved in neurogenesis in prostate cancer, the development of neurofibromas, and breast cancer tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
Uniprot Description
Has growth cone collapse activity against retinal ganglion-cell axons.
Research Articles on SEMA4F
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Product Notes
The SEMA4F sema4f (Catalog #AAA9231972) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SEMA4F Antibody (C-term) reacts with Human, Rat Predicted: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SEMA4F can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000. Researchers should empirically determine the suitability of the SEMA4F sema4f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SEMA4F, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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