Mouse anti-Human, Quail PAX3 Monoclonal Antibody | anti-PAX3 antibody

Mouse Monoclonal [clone C2] (IgG2a) to Quail PAX3

Cat. Num. AAA245842

• Gene Names: PAX3; WS1; WS3; CDHS; HUP2
• Reactivity: Human, Quail
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein G Purified
• Synonyms: PAX3; Monoclonal Antibody; Mouse Monoclonal [clone C2] (IgG2a) to Quail PAX3; Anti-PAX3 Antibody (C-Terminus; clone C2) IHC-plus; CDHS; Paired domain gene 3; Paired box protein Pax-3; Paired domain gene HuP2; Waardenburg syndrome 1; WS1; HUP2; Paired box 3; Paired box homeotic gene 3; WS3; Quail PAX3; anti-PAX3 antibody

• Host: Mouse
• Reactivity: Human, Quail
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: C2
• Purity/Purification: Protein G Purified
• Form/Format: PBS, pH 7.2, 0.09% azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 215

• Applicable Applications for anti-PAX3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Quail
• Immunogen Description: Synthetic peptide derived from a sequence near the carboxy-terminus of quail Pax3.
• Immunogen Type: Synthetic peptide
• Immunogen: PAX3 antibody was raised against synthetic peptide derived from a sequence near the carboxy-terminus of quail Pax3.
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-PAX3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-PAX3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

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NCBI and Uniprot Product Information

• NCBI GI #: 6654638
• NCBI GeneID: 5077
• NCBI Accession #: NP_000429.2
• NCBI GenBank Nucleotide #: NM_000438.5
• Molecular Weight: 55,975 Da
• NCBI Official Full Name: paired box protein Pax-3 isoform PAX3a
• NCBI Official Synonym Full Names: paired box 3
• NCBI Official Symbol: PAX3
• NCBI Official Synonym Symbols: WS1; WS3; CDHS; HUP2
• NCBI Protein Information: paired box protein Pax-3; paired domain gene 3; paired domain gene HuP2; paired box homeotic gene 3
• UniProt Protein Name: Paired box protein Pax-3
• Protein Family: Paired box protein
• UniProt Gene Name: PAX3
• UniProt Synonym Gene Names: HUP2
• UniProt Entry Name: PAX3_HUMAN

NCBI Description

This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. [provided by RefSeq, Jul 2008]

Uniprot Description

PAX3: Probable transcription factor associated with development of alveolar rhabdomyosarcoma. Can bind to DNA as a heterodimer with PAX7. Interacts with DAXX. Belongs to the paired homeobox family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; sequence-specific DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; cell proliferation; muscle development; organ morphogenesis; sensory perception of sound; apoptosis; heart development; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; pigmentation during development; neural tube closure; neuron fate commitment; spinal cord association neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; regulation of somitogenesis; neural crest cell migration

Disease: Waardenburg Syndrome, Type 3; Rhabdomyosarcoma 2; Craniofacial-deafness-hand Syndrome; Waardenburg Syndrome, Type 1