Rabbit anti-Human SELV Polyclonal Antibody | anti-SELV antibody

SELV, CT (SELV, Selenoprotein V) (Azide free) (HRP)

Cat. Num. AAA6345958

• Gene Names: SELENOV; SELV
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SELV; Polyclonal Antibody; CT (SELV; Selenoprotein V) (Azide free) (HRP); Selenoprotein V; anti-SELV antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SELV antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SELV antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-332 amino acids from the C-terminal region of human SELV.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SELV Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SELV antibody detected the SELV protein (arrow).)

Immunohistochemistry (IHC)

(SELV Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SELV Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SELV Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of SELV Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-SELV antibody

This gene encodes a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.

Product Categories/Family for anti-SELV antibody

Antibodies; Selenoprotein

References

Kryukov, G.V., et al. Science 300(5624):1439-1443(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 33186895
• NCBI GeneID: 348303
• NCBI Accession #: NP_874363.1
• NCBI GenBank Nucleotide #: NM_182704.1
• UniProt Accession #: P59797; Q17RG5
• Molecular Weight: 36,800 Da
• NCBI Official Full Name: selenoprotein V isoform 1
• NCBI Official Synonym Full Names: selenoprotein V
• NCBI Official Symbol: SELENOV
• NCBI Official Synonym Symbols: SELV
• NCBI Protein Information: selenoprotein V
• UniProt Protein Name: Selenoprotein V
• Protein Family: Selenoprotein
• UniProt Gene Name: SELENOV
• UniProt Synonym Gene Names: SelV

NCBI Description

This gene encodes a selenoprotein containing a selenocysteine (Sec) residue, which is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is specifically expressed in the testis. It belongs to the SelWTH family, which possesses a thioredoxin-like fold and a conserved CxxU (C is cysteine, U is Sec) motif, suggesting a redox function for this gene. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Apr 2017]

Uniprot Description

May be involved in a redox-related process.