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Immunohistochemistry (IHC) (Anti-SDHAF1 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Goat SDHAF1 Polyclonal Antibody | anti-SDHAF1 antibody

Anti-SDHAF1 Antibody (aa102-115)

Gene Names
SDHAF1; LYRM8
Reactivity
Human
Predicted: Monkey
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Immunoaffinity Purified
Synonyms
SDHAF1; Polyclonal Antibody; Anti-SDHAF1 Antibody (aa102-115); Rabbit Polyclonal to Human SDHAF1; LYRM8; LYR motif-containing protein 8; SDH assembly factor 1; anti-SDHAF1 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Human
Predicted: Monkey
Clonality
Polyclonal
Specificity
Human SDHAF1.
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Sequence Length
115
Applicable Applications for anti-SDHAF1 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
ELISA: 1:16000
IHC-P: 5 ug/ml
WB: 0.3-1 ug/ml
Immunogen
Synthetic peptide C-HDSTGAPETRPDGR from the C-terminus of human SDHAF1 (NP_001036096.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset (86%).
Conjugation
Unconjugated
Epitope
aa102-115
Preparation and Storage
Store at -20 degree C.
Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-SDHAF1 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC) (Anti-SDHAF1 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(SDHAF1 antibody (0.3 ug/ml) staining of Human Cerebellum lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) (SDHAF1 antibody (0.3 ug/ml) staining of Human Cerebellum lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Related Product Information for anti-SDHAF1 antibody
Plays an essential role in the assembly of succinate dehydrogenase(SDH), an enzyme complex (also referred to as respiratory complex II)that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q)to ubiquinol.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,806 Da
NCBI Official Full Name
succinate dehydrogenase assembly factor 1, mitochondrial
NCBI Official Synonym Full Names
succinate dehydrogenase complex assembly factor 1
NCBI Official Symbol
SDHAF1
NCBI Official Synonym Symbols
LYRM8
NCBI Protein Information
succinate dehydrogenase assembly factor 1, mitochondrial
UniProt Protein Name
Succinate dehydrogenase assembly factor 1, mitochondrial
UniProt Gene Name
SDHAF1
UniProt Synonym Gene Names
SDHAF1

NCBI Description

The succinate dehydrogenase (SDH) complex (or complex II) of the mitochondrial respiratory chain is composed of 4 individual subunits. The protein encoded by this gene resides in the mitochondria, and is essential for SDH assembly, but does not physically associate with the complex in vivo. Mutations in this gene are associated with SDH-defective infantile leukoencephalopathy (mitochondrial complex II deficiency).[provided by RefSeq, Mar 2010]

Uniprot Description

Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Promotes maturation of the iron-sulfur protein subunit SDHB of the SDH catalytic dimer, protecting it from the deleterious effects of oxidants. May act together with SDHAF3.

Research Articles on SDHAF1

Similar Products

Product Notes

The SDHAF1 sdhaf1 (Catalog #AAA2401023) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Anti-SDHAF1 Antibody (aa102-115) reacts with Human Predicted: Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's SDHAF1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). ELISA: 1:16000 IHC-P: 5 ug/ml WB: 0.3-1 ug/ml. Researchers should empirically determine the suitability of the SDHAF1 sdhaf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SDHAF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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