Western Blot (WB)
(Western Blot using T9155-75)
Mouse Tubulin, beta Monoclonal Antibody | anti-TUBB antibody
Tubulin, beta (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain, DAQB-47P19.9-001, dJ40E16.7, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB Protein, TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001) (AP)
Reactivity
Bovine, Human, Mouse, Rat
Applications
Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
Tubulin; beta; Monoclonal Antibody; beta (Beta Tubulin; Tubulin beta Polypeptide; Tubulin beta-2 Chain; DAQB-47P19.9-001; dJ40E16.7; M40; MGC117247; MGC16435; OK/SW-cl.56; TUBB; TUBB Protein; TUBB 1; TUBB1; TUBB 2; TUBB2; TUBB 5; TUBB5; XXbac-BCX111D4.2-001) (AP); anti-TUBB antibody
Host
Mouse
Reactivity
Bovine, Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2T16
Specificity
Recognizes bovine beta-Tubulin isotypes I, II, III, IVa, and IVb of, Mr 50kD. No crossreactivity with alpha or gamma tubulin.
Species Crossreactivity: Human, Rat and Mouse. Predicted to have broad species crossreactivity based on sequence homology.
Species Crossreactivity: Human, Rat and Mouse. Predicted to have broad species crossreactivity based on sequence homology.
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Applicable Applications for anti-TUBB antibody
Western Blot (WB)
Application Notes
WB: 0.1-1ug/ml detects beta-Tubulin in RIPA lysates from 3T3/A31, PC-12, Jurkat and A431 cells. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-beta-Tubulin (0.1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
Purified bovine brain tubulin. Epitope recognized by this antibody has been mapped to aa412-430.
Conjugate
AP
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot using T9155-75)
Western Blot (WB)
(Western Blot using T9155-75)
Product Categories/Family for anti-TUBB antibody
References
1. Lee, M.K., et al., Cell Motil. Cytoskel. 17: 118-132, 1990.
Similar Products
Product Notes
The TUBB (Catalog #AAA6241574) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Tubulin, beta (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain, DAQB-47P19.9-001, dJ40E16.7, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB Protein, TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001) (AP) reacts with Bovine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Tubulin, beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 0.1-1ug/ml detects beta-Tubulin in RIPA lysates from 3T3/A31, PC-12, Jurkat and A431 cells. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-beta-Tubulin (0.1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TUBB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tubulin, beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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