Rabbit SDF-1-alpha Polyclonal Antibody | anti-cxcl12 antibody

SDF-1-alpha (Stromal Cell-derived Factor 1, hSDF-1, C-X-C Motif Chemokine 12, Intercrine Reduced in Hepatomas, IRH, hIRH, Pre-B Cell Growth-stimulating Factor, PBSF, CXCL12, SDF1, SDF1A)

Cat. Num. AAA644213

• Gene Names: cxcl12; sdf1; sdf-1; xSDF-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Neutralization Assay
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: SDF-1-alpha; Polyclonal Antibody; SDF-1-alpha (Stromal Cell-derived Factor 1; hSDF-1; C-X-C Motif Chemokine 12; Intercrine Reduced in Hepatomas; IRH; hIRH; Pre-B Cell Growth-stimulating Factor; PBSF; CXCL12; SDF1; SDF1A); Anti -SDF-1-alpha (Stromal Cell-derived Factor 1; anti-cxcl12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SDF-1a. Species Crossreactivity: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.01% thimerosal, 30% glycerol.

• Applicable Applications for anti-cxcl12 antibody: Western Blot (WB), Immunofluorescence (IF), Neutralization
• Application Notes: Suitable for use in Western Blot, Immunofluorescence and Neutralization.; Dilution: Western Blot: 0.5-1ug/ml; Neutralization: 2-10ug/ml
• Immunogen: Recombinant protein corresponding to human SDF-1a expressed in E. coli.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-cxcl12 antibody

SDF-1a (stromal cell-derived factor-a), also known as pre-B-cell growth-stimulating factor (PBSF), is a 70aa CXC chemokine originally cloned from a bone marrow stromal cell line. Targeted deletion of SDF-1 gene resulted in defects of B-cell lymphopoiesis and bone marrow myelopoiesis. SDF-1 has been shown to be chemotactic for lymphocytes. In addition, SDF-1 was recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV-1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV-1 entry.

Product Categories/Family for anti-cxcl12 antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 311771563
• NCBI GeneID: 399030
• NCBI Accession #: NP_001165249.1
• NCBI GenBank Nucleotide #: NM_001171778.1
• UniProt Accession #: Q8UUJ9; Q498K3
• Molecular Weight: 10,701 Da
• NCBI Official Full Name: stromal cell-derived factor 1 isoform alpha
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 12
• NCBI Official Symbol: cxcl12
• NCBI Official Synonym Symbols: sdf1; sdf-1; xSDF-1
• NCBI Protein Information: stromal cell-derived factor 1; C-X-C motif chemokine 12; stromal-derived factor 1; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)
• UniProt Protein Name: Stromal cell-derived factor 1
• Protein Family: Stromal cell-derived factor
• UniProt Gene Name: cxcl12
• UniProt Synonym Gene Names: sdf1; xSDF-1
• UniProt Entry Name: SDF1_XENLA

Uniprot Description

Function: Chemoattractant. Activates the C-X-C chemokine receptor cxcr4 to induce a rapid and transient rise in the level of intracellular calcium ions, and chemotaxis. Signaling with cxcr4 mediates the directional movement of mesodermal cells during gastrulation. Ref.1 Ref.3

Subunit structure: Monomer or homodimer; in equilibrium. Dimer formation is induced by non acidic pH and the presence of multivalent anions, and by binding to cxcr4 or heparin

By similarity.

Subcellular location: Secreted

By similarity.

Tissue specificity: Isoform alpha is expressed throughout the inner deep cells of the blastocoel roof during gastrula stage embryos. Isoform beta is expressed in the developing central nervous system (CNS) of embryos; at stages 21-23, up-regulated in the anterior of the embryo where neural tube segregation occurs. At stages 32-34, expressed in the mid- and hindbrain, otic vesicles and eye, and the dorsal fin, and the embryonic heart at stage 32. At stages 39-40, isoform beta is highly expressed in the proctodeum in the posterior embryo. In the adult, isoform beta is highly expressed in the spleen, kidney, lung, stomach, testis and skeletal muscle. Lower levels are expressed in adult liver and heart. Expression is present at a very low level in adult brain and skin. Ref.1 Ref.3

Developmental stage: Isoform alpha is expressed from the gastrula stage, whereas isoform beta is not expressed until the tailbud stage. Ref.3

Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.