Rabbit anti-Human, Mouse SDCCAG33 Polyclonal Antibody | anti-SDCCAG33 antibody

SDCCAG33, ID (Teashirt Homolog 1, Serologically Defined Colon Cancer Antigen 33, Antigen NY-CO-33, TSH1, TSHZ1) (HRP)

Cat. Num. AAA6498652

• Gene Names: TSHZ1; CAA; TSH1; NY-CO-33; SDCCAG33
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: SDCCAG33; Polyclonal Antibody; ID (Teashirt Homolog 1; Serologically Defined Colon Cancer Antigen 33; Antigen NY-CO-33; TSH1; TSHZ1) (HRP); anti-SDCCAG33 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SDCCAG33. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SDCCAG33 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human SDCCAG33 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of SDCCAG33 Antibody (Center) in mouse kidney tissue lysates (35ug/lane). SDCCAG33 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with SDCCAG33 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 293 cells using SDCCAG33 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-SDCCAG33 antibody

Antibodies; Cancer Markers

References

1. Core, N., Caubit, X., et.al., Dev. Biol. 308(2), 407-420 (2007). 2. Scanlan, M.J., et.al., Int. J. Cancer 76(5), 652-658 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 38201677
• NCBI GeneID: 10194
• NCBI Accession #: NP_005777
• NCBI GenBank Nucleotide #: NM_005786.5
• UniProt Accession #: Q6ZSZ6; O60534; Q4LE29; Q53EU4
• Molecular Weight: 112,705 Da
• NCBI Official Full Name: teashirt homolog 1
• NCBI Official Synonym Full Names: teashirt zinc finger homeobox 1
• NCBI Official Symbol: TSHZ1
• NCBI Official Synonym Symbols: CAA; TSH1; NY-CO-33; SDCCAG33
• NCBI Protein Information: teashirt homolog 1; antigen NY-CO-33; serologically defined colon cancer antigen 33
• UniProt Protein Name: Teashirt homolog 1
• UniProt Gene Name: TSHZ1
• UniProt Synonym Gene Names: SDCCAG33; TSH1
• UniProt Entry Name: TSH1_HUMAN

NCBI Description

This gene encodes a colon cancer antigen that was defined by serological analysis of recombinant cDNA expression libraries. The encoded protein is a member of the teashirt C2H2-type zinc-finger protein family and may be involved in transcriptional regulation of developmental processes. Mutations in this gene may be associated with congenital aural atresia syndrome. [provided by RefSeq, Jan 2012]

Uniprot Description

TSHZ1: Probable transcriptional regulator involved in developmental processes. May act as a transcriptional repressor (Potential). Defects in TSHZ1 are the cause of congenital aural atresia (CAA). A rare anomaly of the ear that involves some degree of failure of the development of the external auditory canal. The malformation can also involve the tympanic membrane, ossicles and middle ear space. The inner ear development is most often normal. Different CAA forms are known. CAA type I is characterized by bony or fibrous atresia of the lateral part of the external auditory canal and an almost normal medial part and middle ear. CAA type II is the most frequent type and is characterized by partial or total aplasia of the external auditory canal. CAA type IIA involves an external auditory canal with either complete bony atresia of the medial part or partial aplasia that ends blindly in a fistula leading to a rudimentary tympanic membrane. CAA type IIB is characterized by bony stenosis of the total length of the external auditory canal. CAA type III involves bony atresia of the external auditory canal and a very small or absent middle-ear cavity. Belongs to the teashirt C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 18q22.3

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding

Biological Process: anterior/posterior pattern formation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; middle ear morphogenesis; soft palate development

Disease: Aural Atresia, Congenital