Western Blot (WB)
(SCYL1 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the SCYL1 antibody detected the SCYL1 protein (arrow).)
Rabbit anti-Human SCYL1 Polyclonal Antibody | anti-SCYL1 antibody
SCYL1, NT (SCYL1, CVAK90, GKLP, NTKL, TAPK, TEIF, TRAP, N-terminal kinase-like protein, Coated vesicle-associated kinase of 90kD, SCY1-like protein 1, Telomerase regulation-associated protein, Telomerase transcriptional element-interacting factor, Teratom
Gene Names
SCYL1; GKLP; NKTL; NTKL; P105; TAPK; TEIF; TRAP; HT019
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SCYL1; Polyclonal Antibody; NT (SCYL1; CVAK90; GKLP; NTKL; TAPK; TEIF; TRAP; N-terminal kinase-like protein; Coated vesicle-associated kinase of 90kD; SCY1-like protein 1; Telomerase regulation-associated protein; Telomerase transcriptional element-interacting factor; Teratom; Teratoma-associated tyrosine kinase; anti-SCYL1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-SCYL1 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
SCYL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SCYL1.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(SCYL1 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the SCYL1 antibody detected the SCYL1 protein (arrow).)
Western Blot (WB)
(SCYL1 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the SCYL1 antibody detected the SCYL1 protein (arrow).)
Immunohistochemistry (IHC)
(SCYL1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SCYL1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(SCYL1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SCYL1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-SCYL1 antibody
SCYL1 forms multimers following transfection into COS-7 cells. SCYL1 forms a 300-kD trimer using crosslinking reagents. Biochemical analysis revealed no phosphorylation or autophosphorylation activity.The 707-amino acid SCYL1 variant, variant 2, localized to centrosomes during mitosis. During interphase, fluorescence-tagged variant 2 localized in the cytoplasm as well as centrosomes. However, at the beginning of mitosis, the fluorescence appeared as a pair of bright nuclear foci that followed centrosome localization throughout mitosis, while maintaining diffuse cytoplasmic labeling. Endogenous variant 2 in HeLa cells showed a similar staining pattern. Centrosomal localization was independent of microtubules.
Product Categories/Family for anti-SCYL1 antibody
References
Tang, Z., et al., Biochem. Biophys. Res. Commun. 324(4):1324-1332 (2004). Kato, M., et al., Genomics 79(6):760-767 (2002). Liu, S.C., et al., Biochim. Biophys. Acta 1517(1):148-152 (2000). van Asseldonk, M., et al., Genomics 66(1):35-42 (2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
86,371 Da
NCBI Official Full Name
N-terminal kinase-like protein isoform B
NCBI Official Synonym Full Names
SCY1-like 1 (S. cerevisiae)
NCBI Official Symbol
SCYL1
NCBI Official Synonym Symbols
GKLP; NKTL; NTKL; P105; TAPK; TEIF; TRAP; HT019
NCBI Protein Information
N-terminal kinase-like protein; SCY1-like protein 1; coated vesicle-associated kinase of 90 kDa; likely ortholog of mouse N-terminal kinase-like protein; telomerase regulation-associated protein; telomerase transcriptional element-interacting factor; telo
UniProt Protein Name
N-terminal kinase-like protein
Protein Family
UniProt Gene Name
SCYL1
UniProt Synonym Gene Names
CVAK90; GKLP; NTKL; TAPK; TEIF; TRAP
UniProt Entry Name
NTKL_HUMAN
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Product Notes
The SCYL1 scyl1 (Catalog #AAA6345471) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SCYL1, NT (SCYL1, CVAK90, GKLP, NTKL, TAPK, TEIF, TRAP, N-terminal kinase-like protein, Coated vesicle-associated kinase of 90kD, SCY1-like protein 1, Telomerase regulation-associated protein, Telomerase transcriptional element-interacting factor, Teratom reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SCYL1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SCYL1 scyl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SCYL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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