Rabbit SCN8A Polyclonal Antibody | anti-SCN8A antibody

SCN8A Antibody - C-terminal region

Cat. Num. AAA3202484

• Gene Names: SCN8A; MED; PN4; CIAT; BFIS5; NaCh6; CERIII; EIEE13; Nav1.6
• Reactivity: Species reactivity: Human; Predicted species reactivity: Cow, Dog, Horse, Mouse, Pig, Rabbit, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: SCN8A; Polyclonal Antibody; SCN8A Antibody - C-terminal region; anti-SCN8A antibody

• Host: Rabbit
• Reactivity: Species reactivity: Human; Predicted species reactivity: Cow, Dog, Horse, Mouse, Pig, Rabbit, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity Purified
• Form/Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Sequence: Synthetic peptide located within the following region: KSSIFSFRGPGRFRDPGSENEFADDEHSTVEESEGRRDSLFIPIRARERR
• Sequence Length: 475

• Applicable Applications for anti-SCN8A antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Homology: Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%
• Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of human SCN8A
• Preparation and Storage: For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Rabbit Anti-SCN8A AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Adult heartObserved Staining: MembranePrimary Antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy2/3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 secProtocol located in Reviews and Data.)

Immunohistochemistry (IHC)

(Rabbit Anti-SCN8A AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Adult liverObserved Staining: MembranePrimary Antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy2/3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 secProtocol located in Reviews and Data.)

Western Blot (WB)

(Host: RabbitTarget Name: SCN8ASample Type: Fetal Heart lysatesAntibody Dilution: 1.0ug/ml)

Western Blot (WB)

(WB Suggested Anti-SCN8A antibody Titration: 1 ug/mLSample Type: Human heart)

Related Product Information for anti-SCN8A antibody

This is a rabbit polyclonal antibody against SCN8A. It was validated on Western Blot

Target Description: This gene encodes a member of the sodium channel alpha subunit gene family. The encoded protein forms the ion pore region of the voltage-gated sodium channel. This protein is essential for the rapid membrane depolarization that occurs during the formation of the action potential in excitable neurons. Mutations in this gene are associated with mental retardation, pancerebellar atrophy and ataxia. Alternate splicing results in multiple transcript variants.

Product Categories/Family for anti-SCN8A antibody

Polyclonal; Various

NCBI and Uniprot Product Information

• NCBI GI #: 295789135
• NCBI GeneID: 6334
• NCBI Accession #: NP_001171455.1
• NCBI GenBank Nucleotide #: NM_001177984.2
• UniProt Accession #: Q9UQD0
• Molecular Weight: 52kDa
• NCBI Official Full Name: sodium channel protein type 8 subunit alpha isoform 2
• NCBI Official Synonym Full Names: sodium voltage-gated channel alpha subunit 8
• NCBI Official Symbol: SCN8A
• NCBI Official Synonym Symbols: MED; PN4; CIAT; BFIS5; NaCh6; CERIII; EIEE13; Nav1.6
• NCBI Protein Information: sodium channel protein type 8 subunit alpha
• UniProt Protein Name: Sodium channel protein type 8 subunit alpha
• Protein Family: Sodium channel protein
• UniProt Gene Name: SCN8A
• UniProt Synonym Gene Names: MED
• UniProt Entry Name: SCN8A_HUMAN

NCBI Description

This gene encodes a member of the sodium channel alpha subunit gene family. The encoded protein forms the ion pore region of the voltage-gated sodium channel. This protein is essential for the rapid membrane depolarization that occurs during the formation of the action potential in excitable neurons. Mutations in this gene are associated with cognitive disability, pancerebellar atrophy and ataxia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

SCN8A: Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. In macrophages and melanoma cells, isoform 5 may participate in the control of podosome and invadopodia formation. Defects in SCN8A are the cause of cognitive impairment with or without cerebellar ataxia (CIAT). A disorder characterized by markedly delayed cognitive and motor development, attention deficit disorder, and cerebellar ataxia. Features include bilateral esophoria, strabismatic amblyopia, unsustained gaze evoked nystagmus on horizontal gaze, ataxic gait, dysmetria in the upper limbs and dysarthria, with normal strength, tone, and reflexes. Defects in SCN8A are the cause of epileptic encephalopathy, early infantile, type 13 (EIEE13). A form of epilepsy characterized by frequent tonic seizures or spasms beginning in infancy with a specific EEG finding of suppression-burst patterns, characterized by high-voltage bursts alternating with almost flat suppression phases. Patients may progress to West syndrome, which is characterized by tonic spasms with clustering, arrest of psychomotor development, and hypsarrhythmia on EEG. EIEE13 is a severe form consisting of early-onset seizures, features of autism, intellectual disability, ataxia, and sudden unexplained death in epilepsy. Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.6/SCN8A subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, sodium; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: voltage-gated sodium channel complex; cell soma; cytoplasmic membrane-bound vesicle; dendrite; integral to membrane; plasma membrane; Z disc

Molecular Function: voltage-gated sodium channel activity; ATP binding

Biological Process: myelination; nervous system development; muscle development; sensory perception of sound; response to toxin; neuromuscular process; sodium ion transport; generation of action potential; adult walking behavior; peripheral nervous system development

Disease: Epileptic Encephalopathy, Early Infantile, 13; Cognitive Impairment With Or Without Cerebellar Ataxia