Human anti-Human Scl-70 Polyclonal Antibody
Scl-70 (Scleroderma, DNA Topoisomerase I)
Reactivity
Human
Applications
ELISA
Purity
Highly PurifiedPurified by double immunodiffusion against rabbit thymus extract.
Synonyms
Scl-70; Polyclonal Antibody; Scl-70 (Scleroderma; DNA Topoisomerase I); Anti -Scl-70 (Scleroderma; anti-Scl-70 antibody
Host
Human
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Undiluted antibody shows a strong Scl-70 precipitin band. Specificity is verified by a precipitin line of identity with the CDC-AF reference serum. This product shows no reactivity at a dilution of 1:100 when tested against purified Sm/RNP, SS-A, SS-B, Sm or Jo-1 antigens in an ELISA system designed to detect all IgG subclasses. Exhibits strong positive in an Anti-Scl-70 ELISA system.
Purity/Purification
Highly Purified
Purified by double immunodiffusion against rabbit thymus extract.
Purified by double immunodiffusion against rabbit thymus extract.
Form/Format
Supplied as a lyophilized powder in PBS, 0.02% sodium azide. Reconstitute with 2ml ddH2O.
Applicable Applications for anti-Scl-70 antibody
ELISA (EL/EIA)
Application Notes
Suitable for use in ELISA.
Immunogen
Sci-70. Autoantibody derived from donor human serum.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Scl-70 antibody
Topoisomerase I changes the topology of DNA via cleavage of single strand only. Suitable for use in DNA conformational analysis and topology as well as DNA repair, drug resistance, cell proliferation and leukemia studies. The double-helical configuration that DNA strands naturally reside in makes them difficult to separate, and yet they must be separated if enzymes are to transcribe the sequences that encode proteins, or if chromosomes are to be replicated. In so-called circular DNA, in which double helical segment is bent around and joined in a circle, the two strands are topologically linked, or knotted. They cannot be separated by any process that does not involve the breaking of strands. Topoisomerases catalyze and guide the unknotting of DNA.
Product Categories/Family for anti-Scl-70 antibody
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Product Notes
The Scl-70 (Catalog #AAA617576) is an Antibody produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Scl-70 (Scleroderma, DNA Topoisomerase I) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Scl-70 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Suitable for use in ELISA. Researchers should empirically determine the suitability of the Scl-70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Scl-70, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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