Goat SCG10 / STMN2 Polyclonal Antibody | anti-SCG10 antibody

Goat Polyclonal to Human SCG10 / STMN2

Cat. Num. AAA242563

• Gene Names: STMN2; SCG10; SCGN10
• Reactivity: Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: SCG10 / STMN2; Polyclonal Antibody; Goat Polyclonal to Human SCG10 / STMN2; Anti-SCG10 / STMN2 Antibody IHC-plus; STMN2; SCGN10; SCG10; Stathmin-like 2; Protein SCG10; Stathmin-2; Human SCG10; anti-SCG10 antibody

• Host: Goat
• Reactivity: Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken
• Clonality: Polyclonal
• Specificity: Human STMN2.
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 179

• Applicable Applications for anti-SCG10 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: IHC-P: (5 ug/ml); WB: (0.3 - 1 ug/ml); Peptide Enzyme-Linked Immunosorbent Assay (1:128000); ; Immunohistochemistry: MBS242563 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS242563 was determined to be 5 ug/ml.
• Target Species: Human
• Immunogen: SCG10 / STMN2 antibody was raised against synthetic peptide AKTAMAYKEK-C from the N-terminus of human STMN2 (NP_008960.2).
• Epitope: aa2-11
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store at -20°C. Avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-STMN2 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western analysis (1 ug/ml) of Human Brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 34850061
• NCBI GeneID: 11075
• NCBI Accession #: NP_008960.2
• NCBI GenBank Nucleotide #: NM_007029.3
• UniProt Accession #: Q93045; O14952; Q6PK68; A8K9M2; G3V110
• NCBI Official Full Name: stathmin-2 isoform 2
• NCBI Official Synonym Full Names: stathmin 2
• NCBI Official Symbol: STMN2
• NCBI Official Synonym Symbols: SCG10; SCGN10
• NCBI Protein Information: stathmin-2; stathmin-like 2; superior cervical ganglion-10 protein; neuron-specific growth-associated protein; superior cervical ganglia, neural specific 10; neuronal growth-associated protein (silencer element)
• UniProt Protein Name: Stathmin-2
• UniProt Gene Name: STMN2
• UniProt Synonym Gene Names: SCG10; SCGN10; Protein SCG10
• UniProt Entry Name: STMN2_HUMAN

NCBI Description

This gene encodes a member of the stathmin family of phosphoproteins. Stathmin proteins function in microtubule dynamics and signal transduction. The encoded protein plays a regulatory role in neuronal growth and is also thought to be involved in osteogenesis. Reductions in the expression of this gene have been associated with Down's syndrome and Alzheimer's disease. Alternatively spliced transcript variants have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Nov 2010]

Uniprot Description

STMN2: a cytoskeletal protein that may play a role in neuronal differentiation, and in modulating membrane interaction with the cytoskeleton during neurite outgrowth. Associated with punctate structures in the perinuclear cytoplasm, axons, and growth cones of developing neurons. Neuron specific.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 8q21.13

Cellular Component: Golgi apparatus; neuron projection; growth cone; membrane; cell soma; perinuclear region of cytoplasm; axon; lamellipodium; cytoplasm; vesicle; endosome

Molecular Function: tubulin binding; protein binding; calcium-dependent protein binding

Biological Process: positive regulation of microtubule depolymerization; negative regulation of microtubule depolymerization; negative regulation of microtubule polymerization