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Western Blot (WB) (Western blot analysis of SCFD1 antibody (C-term) in MCF-7 cell line lysates (35ug/lane). SCFD1 (arrow) was detected using the purified Pab.)

Rabbit anti-Human SCFD1 Polyclonal Antibody | anti-SCFD1 antibody

SCFD1, CT (Sec1 Family Domain-containing Protein 1, Syntaxin-binding Protein 1-like 2, SLY1 Homolog, Sly1p, FKSG23, STXBP1L2, KIAA0917, C14orf163) (Biotin)

Gene Names
SCFD1; SLY1; RA410; SLY1P; STXBP1L2; C14orf163
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
SCFD1; Polyclonal Antibody; CT (Sec1 Family Domain-containing Protein 1; Syntaxin-binding Protein 1-like 2; SLY1 Homolog; Sly1p; FKSG23; STXBP1L2; KIAA0917; C14orf163) (Biotin); anti-SCFD1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SCFD1.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Sequence Length
642
Applicable Applications for anti-SCFD1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:50-1:100
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human SCFD1 (KLH).
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SCFD1 antibody (C-term) in MCF-7 cell line lysates (35ug/lane). SCFD1 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of SCFD1 antibody (C-term) in MCF-7 cell line lysates (35ug/lane). SCFD1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with SCFD1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain tissue reacted with SCFD1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MDA-MB468 cells using SCFD1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of MDA-MB468 cells using SCFD1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-SCFD1 antibody
References
1. Matsuo, N., J. Biol. Chem. 272(26), 16438-16444 (1997).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
72.38
NCBI Official Full Name
sec1 family domain-containing protein 1 isoform a
NCBI Official Synonym Full Names
sec1 family domain containing 1
NCBI Official Symbol
SCFD1
NCBI Official Synonym Symbols
SLY1; RA410; SLY1P; STXBP1L2; C14orf163
NCBI Protein Information
sec1 family domain-containing protein 1
UniProt Protein Name
Sec1 family domain-containing protein 1
UniProt Gene Name
SCFD1
UniProt Synonym Gene Names
C14orf163; KIAA0917; STXBP1L2; Sly1p
UniProt Entry Name
SCFD1_HUMAN

Uniprot Description

SCFD1: involved in vesicular transport between the endoplasmic reticulum and the Golgi. A cytoplasmic, peripheral membrane protein associated with Golgi stacks and the endoplasmic reticulum

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: SNARE complex; endoplasmic reticulum membrane; cis-Golgi network; plasma membrane; Golgi transport complex; Golgi-associated vesicle

Molecular Function: protein binding; syntaxin binding; protein N-terminus binding

Biological Process: regulation of protein transport; protein transport; response to toxin; response to hypoxia; cell morphogenesis; retrograde vesicle-mediated transport, Golgi to ER; post-Golgi vesicle-mediated transport; vesicle docking during exocytosis; phagocytosis

Research Articles on SCFD1

Similar Products

Product Notes

The SCFD1 scfd1 (Catalog #AAA6501124) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SCFD1, CT (Sec1 Family Domain-containing Protein 1, Syntaxin-binding Protein 1-like 2, SLY1 Homolog, Sly1p, FKSG23, STXBP1L2, KIAA0917, C14orf163) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SCFD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:50-1:100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SCFD1 scfd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SCFD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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