Western Blot (WB)
(IL1RL2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the IL1RL2 antibody detected the IL1RL2 protein (arrow).)
Rabbit anti-Human IL1RL2 Polyclonal Antibody | anti-IL1RL2 antibody
IL1RL2 Antibody (Center)
FC~~1:25
Western Blot (WB)
(IL1RL2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the IL1RL2 antibody detected the IL1RL2 protein (arrow).)
Western Blot (WB)
(IL1RL2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the IL1RL2 antibody detected the IL1RL2 protein (arrow).)
Western Blot(WB)
(All lanes : Anti-IL1RL2 Antibody (Center) at 1:8000 dilution Lane 1: HL-60 whole cell lysate Lane 2: U-87 MG whole cell lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot(WB)
(All lanes : Anti-IL1RL2 Antibody (Center) at 1:8000 dilution Lane 1: HL-60 whole cell lysate Lane 2: U-87 MG whole cell lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Flow Cytometry (FC)
(IL1RL2 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC)
(IL1RL2 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC)
(Overlay histogram showing K562 cells stained with MBS9209873 (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9209873, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Flow Cytometry (FC)
(Overlay histogram showing K562 cells stained with MBS9209873 (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9209873, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010)
Nakki, A., et al. BMC Med. Genet. 11 (1), 50 (2010) :
Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)
Solovieva, S., et al. J. Rheumatol. 36(9):1977-1986(2009)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the interleukin 1 receptor family. An experiment with transient gene expression demonstrated that this receptor was incapable of binding to interleukin 1 alpha and interleukin 1 beta with high affinity. This gene and four other interleukin 1 receptor family genes, including interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 1 (IL1RL1), and interleukin 18 receptor 1 (IL18R1), form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. [provided by RefSeq, Jul 2008]
Uniprot Description
IL1RL2: Receptor for interleukin 1 family member 9 (IL1F9). Binding to the agonist leads to the activation of NF-kappa-B. Belongs to the interleukin-1 receptor family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 2q12
Cellular Component: integral to plasma membrane
Molecular Function: interleukin-1, Type I, activating receptor activity; interleukin-1 receptor activity
Biological Process: positive regulation of T cell differentiation; cytokine and chemokine mediated signaling pathway; regulation of inflammatory response; cellular defense response; innate immune response; positive regulation of interleukin-6 production; inflammatory response; signal transduction
Research Articles on IL1RL2
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Product Notes
The IL1RL2 il1rl2 (Catalog #AAA9209873) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 257-286. The IL1RL2 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL1RL2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), ELISA (EIA), Western Blot (WB). WB~~1:1000 FC~~1:25. Researchers should empirically determine the suitability of the IL1RL2 il1rl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL1RL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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