Rabbit anti-Human SCF Polyclonal Antibody | anti-SCF antibody

SCF, NT (Kit Ligand, C-kit Ligand, Stem Cell Factor, Mast Cell Growth Factor, MGF, KITLG) (FITC)

Cat. Num. AAA6498521

• Gene Names: KITLG; SF; MGF; SCF; SLF; DCUA; FPH2; FPHH; KL-1; Kitl; SHEP7; DFNA69
• Reactivity: Human
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: SCF; Polyclonal Antibody; NT (Kit Ligand; C-kit Ligand; Stem Cell Factor; Mast Cell Growth Factor; MGF; KITLG) (FITC); anti-SCF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KITLG.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SCF antibody: ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:1,000; WB: 1:50-1:100; FC/FACS: 1:10-1:50; IHC: 1:10-1:50; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human KITLG (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with KITLG antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of WiDr cells using SCF (KITLG) Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-SCF antibody

Antibodies; Growth Factors; Cytokines

References

1. Young, S.M., Cell. Signal. 19(12), 2572-2581 (2007). 2. Pick, M., Stem Cells 25(9), 2206-2214 (2007). 3. Yasuda, A., Dig. Dis. Sci. 52(9), 2292-2300 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4505175
• NCBI GeneID: 4254
• NCBI Accession #: NP_000890
• NCBI GenBank Nucleotide #: NM_000899.4
• UniProt Accession #: P21583; Q16487; Q68DZ2; Q7M4N8; Q9UQK7; A0AV09; A8K2Q4; B7ZLM4
• Molecular Weight: 18.5 kDa (165 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: kit ligand isoform b
• NCBI Official Synonym Full Names: KIT ligand
• NCBI Official Symbol: KITLG
• NCBI Official Synonym Symbols: SF; MGF; SCF; SLF; DCUA; FPH2; FPHH; KL-1; Kitl; SHEP7; DFNA69
• NCBI Protein Information: kit ligand
• UniProt Protein Name: Kit ligand
• Protein Family: SCF-associated factor
• UniProt Gene Name: KITLG
• UniProt Synonym Gene Names: MGF; SCF; MGF; SCF; sKITLG
• UniProt Entry Name: SCF_HUMAN

NCBI Description

This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]