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Western Blot (WB) (SCEL Antibody (Center) western blot analysis in NCI-H292,A549 cell line lysates (35ug/lane).This demonstrates the SCEL antibody detected the SCEL protein (arrow).)

Rabbit anti-Human SCEL Polyclonal Antibody | anti-SCEL antibody

SCEL, ID (SCEL, Sciellin) (APC)

Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SCEL; Polyclonal Antibody; ID (SCEL; Sciellin) (APC); Sciellin; anti-SCEL antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Sequence Length
688
Applicable Applications for anti-SCEL antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
SCEL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247-277 amino acids from the Central region of human SCEL.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SCEL Antibody (Center) western blot analysis in NCI-H292,A549 cell line lysates (35ug/lane).This demonstrates the SCEL antibody detected the SCEL protein (arrow).)

Western Blot (WB) (SCEL Antibody (Center) western blot analysis in NCI-H292,A549 cell line lysates (35ug/lane).This demonstrates the SCEL antibody detected the SCEL protein (arrow).)

Flow Cytometry (FC/FACS)

(SCEL Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (SCEL Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-SCEL antibody
The protein encoded by this gene is a precursor to the cornified envelope of terminally differentiated keratinocytes. This protein localizes to the periphery of cells and may function in the assembly or regulation of proteins in the cornified envelope. Transcript variants encoding different isoforms exist. A transcript variant utilizing an alternative polyA signal has been described in the literature, but its full-length nature has not been determined.
Product Categories/Family for anti-SCEL antibody
References
Pyle, A.L., et al. Cardiovasc. Pathol. 19 (2), E13-E20 (2010): Corona, W., et al. Anticancer Res. 24 (3A), 1417-1419 (2004): Champliaud, M.F., et al. J. Invest. Dermatol. 121(4):781-785(2003). Champliaud, M.F., et al. Genomics 70(2):264-268(2000). Champliaud, M.F., et al. J. Biol. Chem. 273(47):31547-31554(1998).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
sciellin isoform 1
NCBI Official Synonym Full Names
sciellin
NCBI Official Symbol
SCEL
NCBI Protein Information
sciellin
UniProt Protein Name
Sciellin
Protein Family
UniProt Gene Name
SCEL
UniProt Entry Name
SCEL_HUMAN

NCBI Description

The protein encoded by this gene is a precursor to the cornified envelope of terminally differentiated keratinocytes. This protein localizes to the periphery of cells and may function in the assembly or regulation of proteins in the cornified envelope. Transcript variants encoding different isoforms exist. A transcript variant utilizing an alternative polyA signal has been described in the literature, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

SCEL: May function in the assembly or regulation of proteins in the cornified envelope. The LIM domain may be involved in homotypic or heterotypic associations and may function to localize sciellin to the cornified envelope. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 13q22

Cellular Component: cytoplasm; cornified envelope

Molecular Function: protein binding; zinc ion binding

Biological Process: keratinocyte differentiation; epidermis development; embryonic development

Research Articles on SCEL

Similar Products

Product Notes

The SCEL scel (Catalog #AAA6345262) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SCEL, ID (SCEL, Sciellin) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SCEL can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SCEL scel for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SCEL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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