Rabbit SCAMP1 Polyclonal Antibody | anti-SCAMP1 antibody

SCAMP1 Polyclonal Antibody

Cat. Num. AAA3006197

• Gene Names: SCAMP1; SCAMP; SCAMP37
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SCAMP1; Polyclonal Antibody; SCAMP1 Polyclonal Antibody; Secretory carrier-associated membrane protein 1; Secretory carrier membrane protein 1; SCAMP; anti-SCAMP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SCAMP1 polyclonal antibody detects endogenous levels of SCAMP1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 338

• Applicable Applications for anti-SCAMP1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 293-338 of Human SCAMP1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SCAMP1 polyclonal antibody at 1:500 dilutionLane1:A549 cell lysateLane2:Raw264. 7 cell lysateLane3:PC12 cell lysate)

Related Product Information for anti-SCAMP1 antibody

Secretory carrier membrane proteins (SCAMPs) are components of the post Golgi membranes and are invovled in endocytosis, vesicle recycling and membrane trafficking. The structural features of SCAMPs include multiple N-terminal NPF repeats and four highly conserved transmembrane regions. These NPF repeats frequently interact with EH domain proteins and aid in the budding of transport vesicles from the plasma membrane or the Golgi complex. Endocytic budding at the plasma membrane and vesicle budding at the trans-Golgi complex facilitates binding of SCAMP proteins to EH domain proteins. SCAMPs exist as distinct but related proteins that include SCAMP1, SCAMP2, and SCAMP3. Tyrosine-phosphorylation by the epidermal growth factor-receptor of SCAMP1 and SCAMP3 suggests that SCAMPs are regulated by phosphorylation. Although SCAMPs are ubiquitously expressed throughout all tissue, in neural tissue the synaptic vesicles express a particularly high concentration of SCAMP1.

NCBI and Uniprot Product Information

• NCBI GI #: 116256358
• NCBI GeneID: 9522
• NCBI Accession #: NP_004857.4
• NCBI GenBank Nucleotide #: NP_004857.4
• UniProt Accession #: O15126
• Molecular Weight: ~ 37kDa
• NCBI Official Full Name: secretory carrier-associated membrane protein 1 isoform 1
• NCBI Official Synonym Full Names: secretory carrier membrane protein 1
• NCBI Official Symbol: SCAMP1
• NCBI Official Synonym Symbols: SCAMP; SCAMP37
• NCBI Protein Information: secretory carrier-associated membrane protein 1
• UniProt Protein Name: Secretory carrier-associated membrane protein 1
• Protein Family: Secretory carrier-associated membrane protein
• UniProt Gene Name: SCAMP1
• UniProt Synonym Gene Names: SCAMP; Secretory carrier membrane protein 1
• UniProt Entry Name: SCAM1_HUMAN

NCBI Description

This gene product belongs to the SCAMP family of proteins, which are secretory carrier membrane proteins. They function as carriers to the cell surface in post-golgi recycling pathways. Different family members are highly related products of distinct genes, and are usually expressed together. These findings suggest that these protein family members may function at the same site during vesicular transport rather than in separate pathways. A pseudogene of this gene has been defined on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

SCAMP1: Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface. Belongs to the SCAMP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: zymogen granule membrane; clathrin-coated vesicle; synaptic vesicle membrane; recycling endosome membrane; integral to membrane; trans-Golgi network

Molecular Function: protein domain specific binding; protein binding

Biological Process: protein transport; exocytosis; endocytosis; post-Golgi vesicle-mediated transport