Rabbit SATB1 Polyclonal Antibody | anti-SATB1 antibody

SATB1 Polyclonal Antibody

Cat. Num. AAA3013405

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: SATB1; Polyclonal Antibody; SATB1 Polyclonal Antibody; DNA binding protein SATB1; DNA-binding protein SATB1; SATB homeobox 1; Special AT rich sequence binding protein 1 (binds to nuclear matrix/scaffold associating DNA); Special AT rich sequence binding protein 1; Special AT-rich sequence-binding protein 1; anti-SATB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SATB1 polyclonal antibody detects endogenous levels of SATB1 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 763

• Applicable Applications for anti-SATB1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1,000; ICC: 1:50-1:200; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of SATB1 on human thymus lysates using anti-SATB1 antibody at 1/1,000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-SATB1 antibody. Counter stained with hematoxylin.)

Related Product Information for anti-SATB1 antibody

The homeoproteins CCAAT displacement protein (CDP) and special AT-rich sequence binding protein 1 (SATB1) are transcriptional repressors of many cellular genes, and they participate in cell development and cell type differentiation. SATB1 is expressed primarily in thymocytes, and, like CDP, it also contains a distinct homeobox DNA-binding domain that is essential for DNA binding. SATB1 and CDP interact through these homeodomains and synergistically function as mediators of gene expression. SATB1 contains an additional domain that has a higher affinity for DNA and specifically facilitates the direct association between SATB1 and the nuclear matrix attachment regions (MARs) of DNA. MARs are specific DNA sequences that bind to the nuclear matrix and form the base of chromosomal loops that organize the chromosomes and regulate DNA transcription and replication within the nucleus. The association of SATB1 with the core unwinding element within the base-unpairing region of MARs requires both the MAR and homeobox binding domains of SATB1.

NCBI and Uniprot Product Information

• NCBI GI #: 196114999
• NCBI GeneID: 6304
• NCBI Accession #: NP_001124482.1
• NCBI GenBank Nucleotide #: NP_001124482.1
• UniProt Accession #: Q01826; Q60611
• Molecular Weight: 100kDa
• NCBI Official Full Name: DNA-binding protein SATB1 isoform 1
• NCBI Official Synonym Full Names: SATB homeobox 1
• NCBI Official Symbol: SATB1
• NCBI Protein Information: DNA-binding protein SATB1
• UniProt Protein Name: DNA-binding protein SATB1
• Protein Family: DNA-binding protein
• UniProt Gene Name: SATB1
• UniProt Entry Name: SATB1_HUMAN

NCBI Description

This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. [provided by RefSeq, Apr 2016]