Rabbit anti-Human, Mouse SARM Polyclonal Antibody | anti-SARM1 antibody

SARM Antibody

Cat. Num. AAA150071

• Gene Names: SARM1; SARM; SAMD2; MyD88-5
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: SARM Antibody is affinity chromatography purified via peptide column.
• Synonyms: SARM; Polyclonal Antibody; SARM Antibody; SAMD2; MyD88-5; KIAA0524; Sterile alpha and TIR motif-containing protein 1; Sterile alpha and Armadillo repeat protein; sterile alpha and TIR motif containing 1; anti-SARM1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: SARM Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 724

• Applicable Applications for anti-SARM1 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: SARM antibody can be used for detection of SARM by Western blot at 0.5 to 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 2 mug/mL. For immunofluorescence start at 2 mug/mL.
• Immunogen: SARM antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human SARM.; The immunogen is located within amino acids 640-690 of SARM.
• Buffer: SARM Antibody is supplied in PBS containing 0.02% sodium azide.
• Homology: Predicted species reactivity based on immunogen sequence: Pig: (86%)
• Preparation and Storage: SARM antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of SARM in Daudi cell lysate with SARM antibody at (A) 0.5 and (B) 1 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry staining of Daudi cells using SARM antibody at 2 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of SARM in Daudi cells with SARM antibody at 2 μg/mL.)

Related Product Information for anti-SARM1 antibody

SARM Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. SARM (SAM and ARM-containing protein), along with other molecules such as TIRP, TRIF, TIRAP, and MyD88, is thought to serve as an adaptor protein for the TLRs that allows for the activation of downstream kinases and NF-κB, and ultimately the expression of proteins involved in host defense. While SARM has not been conclusively shown to associate directly with TLRs, the presence of a Toll-interluekin-1 (TIR) domain in SARM is consistent with a role as a signaling molecule.

NCBI and Uniprot Product Information

• NCBI GI #: 154090976
• NCBI GeneID: 23098
• NCBI Accession #: NP_055892
• NCBI GenBank Nucleotide #: NM_015077.3
• UniProt Accession #: Q6SZW1; O60277; Q7LGG3; Q9NXY5
• Molecular Weight: Predicted MW: 80 kDa; Observed MW: 85kDa
• NCBI Official Full Name: sterile alpha and TIR motif-containing protein 1
• NCBI Official Synonym Full Names: sterile alpha and TIR motif containing 1
• NCBI Official Symbol: SARM1
• NCBI Official Synonym Symbols: SARM; SAMD2; MyD88-5
• NCBI Protein Information: sterile alpha and TIR motif-containing protein 1; tir-1 homolog; SAM domain-containing protein 2; sterile alpha and Armadillo repeat protein; sterile alpha motif domain-containing protein 2; sterile alpha and HEAT/Armadillo motif protein, ortholog of Drosophila
• UniProt Protein Name: Sterile alpha and TIR motif-containing protein 1
• Protein Family: Sterile alpha and TIR motif-containing protein
• UniProt Gene Name: SARM1
• UniProt Synonym Gene Names: KIAA0524; SAMD2; SARM; MyD88-5; SAM domain-containing protein 2
• UniProt Entry Name: SARM1_HUMAN

Uniprot Description

SARM1: Involved in innate immnune response. Acts as a negative regulator of TICAM1/TRIF-dependent Toll-like receptor signaling by inhibiting induction of TLR3- and TLR4-dependent genes. Specifically blocks TICAM1/TRIF-dependent transcription-factor activation and gene induction, without affecting the MYD88- dependent pathway or non-TLR signaling. Negative regulator of NF- kappa-B and IRF activation. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 17q11

Cellular Component: microtubule; mitochondrion; axon; cytoplasm; dendrite; extrinsic to mitochondrial outer membrane; synapse; cytosol; cell junction

Biological Process: regulation of apoptosis; MyD88-independent toll-like receptor signaling pathway; regulation of dendrite morphogenesis; response to glucose stimulus; toll-like receptor signaling pathway; innate immune response; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway