Mouse VEGFA Monoclonal Antibody | anti-VEGFA antibody

VEGFA antibody

Cat. Num. AAA838569

• Gene Names: VEGFA; VPF; VEGF; MVCD1
• Applications: Immunohistochemistry, Western Blot
• Purity: VEGFA antibody was purified by affinity chromatography.
• Synonyms: VEGFA; Monoclonal Antibody; VEGFA antibody; Monoclonal VEGFA; Anti-VEGFA; Vascular endothelial growth factor A; VPF; VEGF; Folliculostellate cell-derived growth factor; Glioma-derived endothelial cell mitogen; anti-VEGFA antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 2F7
• Purity/Purification: VEGFA antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 137

• Applicable Applications for anti-VEGFA antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50; WB: 1:1000
• Biological Significance: Vascular Endothelial Growth Factor (VEGFA) is a potent mitogen for vascular endothelial cells. It significantly influences vascular permeability and is a strong angiogenic protein. VEGF probably also plays a role in neovascularization under physiological conditions. Due to its influences on vascular permeability, VEGF may be involved in altering blood-brain-barrier functions under normal and pathological conditions.
• Cross-Reactivity: Human
• Immunogen: VEGFA antibody was raised in mouse using a recombinant protein expressed in E Coli corresponding to amino acids 27-233 of human VEGF as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of VEGFA protein in paraffin embedded Adenocarcinoma of Human breast tissue using VEGFA antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant VEGFA protein (Right) or empty vector (Left) detected with VEGFA antibody)

Related Product Information for anti-VEGFA antibody

Mouse monoclonal VEGFA antibody

Product Categories/Family for anti-VEGFA antibody

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 284172473
• NCBI GeneID: 7422
• NCBI Accession #: NP_001165101.1
• NCBI GenBank Nucleotide #: NM_001171630.1
• Molecular Weight: 34,406 Da
• NCBI Official Full Name: vascular endothelial growth factor A isoform p
• NCBI Official Synonym Full Names: vascular endothelial growth factor A
• NCBI Official Symbol: VEGFA
• NCBI Official Synonym Symbols: VPF; VEGF; MVCD1
• NCBI Protein Information: vascular endothelial growth factor A
• UniProt Protein Name: Vascular endothelial growth factor A
• UniProt Gene Name: VEGFA
• UniProt Synonym Gene Names: VEGF; VEGF-A; VPF
• UniProt Entry Name: VEGFA_HUMAN

NCBI Description

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.