Rabbit SAP18 Polyclonal Antibody | anti-SAP18 antibody

SAP18 Rabbit Polyclonal

Cat. Num. AAA7603829

• Gene Names: SAP18; SAP18P; 2HOR0202
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: SAP18; Polyclonal Antibody; SAP18 Rabbit Polyclonal; anti-SAP18 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 153

• Applicable Applications for anti-SAP18 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Immunogen: Sin3A-associated protein, 18kDa
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using MBS7603829 (SAP18 antibody) at dilution of 1:50)

Western Blot (WB)

(K-562 cells were subjected to SDS PAGE followed by western blot with MBS7603829 (SAP18 antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 48146187
• NCBI GeneID: 10284
• UniProt Accession #: O00422; Q2TTR4; Q6IAW9; Q8N606; Q9UF14; B2R494
• Molecular Weight: 17,561 Da
• NCBI Official Full Name: SAP18
• NCBI Official Synonym Full Names: Sin3A associated protein 18
• NCBI Official Symbol: SAP18
• NCBI Official Synonym Symbols: SAP18P; 2HOR0202
• NCBI Protein Information: histone deacetylase complex subunit SAP18
• UniProt Protein Name: Histone deacetylase complex subunit SAP18
• Protein Family: Zinc finger A20 domain-containing stress-associated protein
• UniProt Gene Name: SAP18
• UniProt Entry Name: SAP18_HUMAN

NCBI Description

Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Dec 2008]

Uniprot Description

SAP18: Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Belongs to the SAP18 family.

Chromosomal Location of Human Ortholog: 13q12.11

Cellular Component: cytoplasm; histone deacetylase complex; nuclear speck; nucleoplasm

Molecular Function: histone deacetylase activity; protein binding; transcription corepressor activity

Biological Process: histone deacetylation; mRNA processing; negative regulation of nuclear mRNA splicing, via spliceosome; positive regulation of apoptosis; regulation of alternative nuclear mRNA splicing, via spliceosome; regulation of transcription from RNA polymerase II promoter; RNA splicing; transcription, DNA-dependent